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Author Topic: Agrobacterium rhizogenes -- How to obtain?  (Read 203 times)

Vesp

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Re: Agrobacterium rhizogenes -- How to obtain?
« Reply #20 on: September 20, 2010, 12:34:22 PM »
You should do that!
I'll have to lookup AGL1 when I have time... What is it?
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chaotic_mind

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Re: Agrobacterium rhizogenes -- How to obtain?
« Reply #21 on: September 21, 2010, 09:56:15 AM »
Its had most of the genes involved in tumor formation removed but it can still infect things. You put a plasmid in it with your gene of interest in it between a left and right boarder of the T-DNA which is then inserted into the plant genome randomly. its actually Agrobacterium tumefaciens.

hxxp://en.wikipedia.org/wiki/Agrobacterium_tumefaciens

Vesp

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Re: Agrobacterium rhizogenes -- How to obtain?
« Reply #22 on: September 21, 2010, 12:20:50 PM »
I see, that is what I thought might have been the case.
It is a shame that your strain is so hard to get, since it obviously can't be isolated from the wild it needs to be had via university.  :(

Is it at all regulated? Since it doesn't have the tumor gene in it, I bet that it is not.
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