Serotonin/Creatin-Sulfate???

[Chemikaze]

Bees,do you have any ideas on separation of serotonin and creatin(Glycine, N-(aminoiminomethyl)-N-methyl).
May be a bee with Merck could help? .
I guess Creatin is very soluble in water ...hmm well serotonin should be too ?
When I die bury me upside down so the world can kiss my ass.

[Lilienthal]

Are you sure you don't have a serotonin-creatinine-sulfate complex?

Creatinine is soluble in 12 parts water, slightly soluble in ethanol, practically insoluble in acetone, ether, chloroform.

Maybe basify the complex with ammonium hydroxide solution and extract with chloroform or methylene chloride?

[Chemikaze]

Hmm,i guess it IS a complex,but is it creatinine or creatin because they are absoulutely different substances,or may be you mistyped Lilienthal?
SWIH are you reading this,is it creatine or creatinine you`ve got?

When I die bury me upside down so the world can kiss my ass.

[Hecata]

well, i have serotonine + creatine. Not creatinine.

So, what we need - solubility of cretine and serotonine in some different liquids. If Lilienthal "creatinine" creatine , i need to know solubility of serotonine in acetone, chloroform and ether.
Tongue-tied and twisted just an earth-bound misfit, I

[Lilienthal]

Under neutral or basic conditions there is an equilibrium between creatine and creatinine, while under acidic conditions there's only creatinine.

Under basic conditions the carboxy group should be deprotonated, making the molecule extremely hydrophilic compared to serotonine freebase. You can't use a stronger base than NH₄OH because that would degrade the serotonin.

Creatine:

Molecule:

creatinine ("NC(=N)N(C)CC(=O)O")



Creatinine is the cyclized form:

Molecule:

creatinine ("C1C(=O)NC(=N)N1C")

[Hecata]

It's a beautiful idea. Tnx, we try it.
Tongue-tied and twisted just an earth-bound misfit, I