Just throw some contaminated donuts, and they will beat a path to your lab door

Who wants to start a collection of gangrene-rotted trotters, pickled in formalin?

Easy to get too, at least in the UK, it is available OTC in the form of eye drops.

Of course a new bottle will needs be sterile too, what with it being intended to be dropped into one's eyes.

'nother question, I read a paper noting the use of fluorescein as a stain for fluorimetric analysis, the ergot alkaloids fluorescing a different color than the background fluorescence of fluorescein, looks an interesting method for analytical work, and accessible to the amateur/hobbyist/clandestine chemist/mixture of the above.

As it so happens, I happen to have..erm...acquired a box of fluorets, which are eye staining paper strips containing 1mg of fluorescein each, localised in a small tab on the end of the strip, what are suitable solvents for it, I think they could be extracted easily, and as only dilute concentrations would be needed to stain a small sample of agar from a colonized plate, 100mg might be quite a bit, which is give or take a couple of strips that may have been used by the former owner.

i think that method is superior to solvent extraction because there are reports of metal catylyzed oxidations and it gets very toucy because of all the trace metals used in broths

AFAIK the 4-DMAB reacts with indoles to form a dimeric structure of two indolic molecules bridged by the 4-DMAB, which I believe to be a more strongly chromophoric species.

Wow, this is very exciting Overunity!

 Fantastic stuff, complete and comprehensive overunity! thanx for sharing the love

  I would suggest some Nicotinamide aka vitamin B3, and some 5-HTP(hydroxytryptophan) as worth adding to the nutrient, they are both indolic compounds, and couldnt hurt

   f1

Yes - this is more so about taking out the variables than adding additional ones which may cause different problems.

My big problem with the 5-HTP is that OH on the indole. Why?

-Because according to several search engine 5-hydroxy-LSA/LSD or any kind of lysergamides has been never synthetised/used. And not even the methoxy/ethoxy compounds.

-If the C.Paspali or any other fungus used in the bioreactor would make a new (lysergic) compound with the 5-HTP than how could the home chemist identify his compound without any reference? It's like in 18' century chemistry, we add something, we get out something and we hope that there is something in it what we want.

-The 5-th position in tryptamine chemistry is like a magical switch, it could easily turn out that a highly toxic or a 10, 100x "stronger" compound will come out from the reactor. Or something what is completely useless

-And afterall LSA is an unstable compound, it could decompose to a black tar easily. I have a parrot who had worked with 5-MeO indole, 5-Br indole, 5-OH indole and normal indole a lot and usually the indole was white, 5-Br indole was a bit gray, the 5-MeO indole was a brownish goo and the 5-OH indole was a black goo, while they (except the 5-Br) should be a white crystalline solid. The 5-MeO, 5-OH indole could be easily decomposed into a black tar, because they are highly sensitive to enerything (heat, oxidation ect.), so the 5-OH LSA should behave simmilar.


I think it's not worth thinking of the 5-HTP.

But, normal indole is good for the reaction:
From: Patent number: US2936266

The fungus was cultured in a small fermenting tank 5 litres capacity at 27 C The tank was equipped means of aeration ceramic candles agitator and removing equipment pH regulator and sterile.
3.00% glucose
0.06% urea
0.40% hydrolised casein
0.008% KH2P04
0.01% MgS04 7H20
Plus trace elements

Culture conditions Air induction 200 litres per litre of substrate per hour agitator speed 400 rpm. Inoculation with mycelium taken from parent culture.
 During the growth phase the pH value was maintained within the range of pH 4.8 to 5.0 At the end of 97 hours the available sugar had been assimilated and growth completed. At this stage the mycelium contained 19.7 fat The presence of alkaloids could not be traced either in the mycelium or in the substrate.
Additions of 0.01% indole and 0.005% KCN were then made. Aeration was stopped but agitation continued. The pH value was main tained at 5.8 to 6.0 The production of alkaloids in the substrate and in the mycelium rapidly rose and passed through an optimum in the 136th hour when the total alkaloid content in the mycelium and in the substrate calculated on the basis of ergometrine was determined at 0.13 g per litre.

And here is something from another patent (US3038840):

The cultivation is carried out with the following nutrient medium (1000ml)
Mannitol 4 %
Glucose 1 %
Succinic acid 2%
KH2P04 0.1 %
MgS04 7H20 0.03 %
Chick pea meal 0.5 %
Distilled water

The pH is adjusted to 5.2 with aqueous ammonia solution. The fermentation is carried out according the the procedure described in Example 1. After 7-9 days incubation the production of alkaloids reaches to 1400 1600 microgram/ml (1.4-1.6g/litre).
Other suitable nitrogen sources are soyabean meal peanut meal bean meal lentil meal pea meal potato meal hydrolyzed casein yeast extract corn steep liquor and the like.

 Thanx anirecetam,  I admit I was only having a "guess" but my guess was based on comments made by sasha in TIHKAL 

   with luck, a small scale reactor will come online after xmas, when the 'grasses are seeded, and fungal"

  You will be kept in the loop 

   Regards

   f1 

sry for the minirant, just frustrated with the plethora of theories and patent-posting but lack of results

 NO worries matey, I hear you loud and clear...

I realize not everyone has access to HPLC or LCMS, but p-DMAB is OTC as are TLC  plates. regurgitating old papers and patents are of little use (some of the more prolific wasps have already posted a s#itload of great refs in other threads), we need more applied results, even if it's just qualitative analysis.

 So true, I'd just like to say, its a rockin topic/post you started! I'd like to ask more about the photos, but maybe thats best done via pm, just so no 'site rules' are broken, accidentally or otherwise!

 Keep up the good work nootropic dude

 f1