five-fold increase in total alkaloid production

[Bubbleplate]

An article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY describes a technique to increase alkaloid yield in Claviceps culture by adding Perfluorocarbons to the media. Perflurocarbons are being investigated and used for, among other things, synthetic blood.
If someone has access to and could post this article, it would be greatly appreciated!
Title:   Application of oxygen vectors to Claviceps purpurea cultivation
Author/s:   M Menge, J Mukherjee, T Scheper
Source   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,55(4):411-416 2001
ISSN   01757598    
Publisher  SPRINGER-VERLAG NEW YORK, INC.

Abstract:  The application of a two-phase fermentation system for the production of ergot peptide alkaloids by Claviceps purpurea is described. Perfluorocarbons (PFC) are used as oxygen vectors in Claviceps fermentation for the first time. In shake-flask cultivations, the inclusion of PFC in the medium brings about a five-fold increase in the total alkaloid production and a six-fold increase in the pharmaceutically important component, ergotamine.

[Rhodium]

Full Paper:

Appl. Microbiol. Biotechnol. 55, 411-416 (2001)

(https://www.thevespiary.org/rhodium/Rhodium/pdf/claviceps.purpurea.oxygen.vectors.pdf) DOI:

10.1007/s002530100592

[Bubbleplate]

minimal or no increase using oxygen carriers.
Pacifici ("Production of Lysergic Acid Derivatives in Submerged Culture") found that the addition of 50 mg. L-Tryptophan & 1 gram Acetamide per liter of media increased alkaloid concentration 500% vs. no supplement.

[Bandil]

Bubbleplate> Are you sure it was L-tryptophan that caused the increased production? As far as i remember it was the dl- version. I don't have the article here, so i can't be sure of course.

[Bubbleplate]

but I'm looking at a hard copy in my hands and it says:
"50 mg. L-tryptophan + 1 g. Acetamide".
Also, throughout the article, it states that "l-tryptophan" was used.
When I ordered Tryptophan, I never though to specify the type, and when it arrived, it was L-Tryptophan. Maybe that is the common type?