I think that you have to read a bit more about the theory behind the chromatography before you ask such questions. Don't get me wrong, it's just that you seem to have overlooked the importnant details regarding basic principles of the subject, for instance there is no universal solvent-system. Sometimes you have to increase the polarity, sometimes you have to decrease it, depending on the nature of the substances you want to separate.
Take a look at the Vogel's third edition at the rhodium.ws, I think that it has a chromatography chapter.