God, don't take me too serious (except my statement that I am an atheist). Milking frogs is cruel. And besides, A/Bing blenderized frogs results in the the worst emulsions imaginable. Yuck!
Hest, I know that there are just a few chemicals needed to make shitloads of DMT but some of those are dangerous, others are difficult to obtain (LAH!) and DMT synthesis is not doable in an apartment (neighbours do not like it).
But biosynthesis is simple, on the condition that you use the right organism. A cubensis (and a paspali! - perhaps most fungi) only need a mixture of 6g of maltose or dextrose, 500mg of British Marmite, 1cc of household 3% hydrogen peroxide and 100ml of water to produce 2000-2500mg of dry mycelial biomass at roomtemperature in a marmelade jar or tupperware container (etc.) as surface culture without stirring. The peroxide enables inoculation without any sterile precaution (just mix the ingredients except the H2O2 in a milkpan, heat until it begins to boil, then add H2O2, pour in jar and allow to cool down. Now add a bit of mycelium which has been cultured out on a bit of colonized sawdust/cardboard which was soaked in the same mixture (other than bits of agar, pieces of sawdust/cardboard float on the mixture). Do A/B after the medium has colonized to get your crude alkaloids (in this way the A/B is possible in the same container as the fermentation!). I think I dare to guarantee/bet that you won't find any medium which gives you more biomass or a stronger blueing / pink decolorization of named mycelia. The secret is in the marmite. Malt sugar is the best energy source for fungi (I have tried all sugars/meals/flours I could find and didn't find a better mixture for liquid media than malt+marmite).
So I hope you see why I put my cards on liquid fermentation. What is needed are the right organisms.
A modified cubensis may still be theory, but how close can we approach our goals with other life forms? Schizophylum commune (the split gill fungus, perhaps the
world's most widespread fungus) produces indole. There may even be a direct source for DMT: a Phalaris callus culture, grown out as biomass in separate cells in a liquid medium. I still have to collect literature to learn how that should be done. No idea how much callus biomass is needed for a good dose, but I understand that Phalaris seedlings contain more DMT compared to bigger plants so this method at least has some potential. Especially since the whole cultivation process is both OTC and legal to do.
MHRB? Yeah. It is the best choice of the moment. But I suspect that the prices will rise tremendously once ppl discover how easy it is to get the goodies out (do you beez know the black market price of purified nigerine?). Cultivation of MHRB is a slow process and not suitable for temperate regions.
bibliopharmacophile