Quantitative and Qualitative TLC Analysis of 5meo DMT
By ClearLight and Desert Sun
We are reporting here our method for qualitative and quantitative analysis of the titled compounds using prior hive posts in addition to a unique photometric method for quantitative analysis of UV and visualized chromaphores.
Using TLC plates we have determined the rF values of the titled compounds and identified their wet and dry chromaphores using the modified Van Urk/Salkowski reagent system as well as their respective concentrations.
(see link for full pdf document)
http://www.angelfire.com/blog/clearlight/TLC_Analysis_of_5meo_DMT.pdf
Conclusions:
Qualitative measurements of TLC plates are now possible using simple, home equipment. Utilizing a free program, Image, background subtracted image density (BSID) measurements, when averaged, correlate very well with the loadings on the plate. Use of a wet plate will not provide accurate measurements but will provide some qualitative information as to the nature of the materials under analysis. A 5 microliter spot containing 25 micrograms of the title compound was compared to a 10 microliter spot containing 50 micrograms of the title compound. The results for both U.V and visualized BSID measurements were 1.99 and 2.09 respectively.
Short Wave UV measurements of the image as well as dry visualized measurements provide accurate information as to the total amount of compound present when compared to a standard. This method now provides the experimenter with a rapid, easy way to quantify the amounts of compounds present in a sample and may be correlated to other compounds of interest to provide rapid, accurate measurements of your materials. This system compares favorably to analysis done using $20k –30k systems.