The Vespiary
The Hive => Tryptamine Chemistry => Topic started by: alice_d25 on June 13, 2000, 06:58:00 PM
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Does anyone have any details on where to find Claviceps Paspali?
Does it grow in commercial cultivars (Wheat/oats/rye/etc), and is it present in the soil etc. Has anyone found this in OZ, and if so, in which areas?
I have some idea of what it looks like, now I have to find it!!
Does it grow in the grain after harvest, (ie in the silos?) or is it only while the plant is growing? Once I have a culture can I grow it in a Lab environment, and if so, what would be the best culture medium?
Is it true that it grows on the surface of mashed weetbix when they are made up & left in cool place for X weeks?
nb Is it possible to make Lysergic acid from LSA (Morning Glory seeds etc) by Alkaline Hydrolysis?
Yours
Alice D25
"Trust in God, but tie your Camel" [Arab Proverb]
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I would like to know the same thing. Most of the symth. for LSD I have read are very hard for the average shade tree Chemist to understand. I would like to see someone do a writeup like Cheapskate did with Earth Wind and Fire give a down to earth way to make all of the precursers step by step with the easiest to find material to make your own home made LSD. It can't be any harder to make then Ludes. Rock On!
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There is no easy way to make LSD. If you don't know how to produce LSA (its just a carboxylic acid man, how do we build carboxylic acids? Gringard! MgX that is) than just buy the shit, don;t bother making it, as you will poison or kill yourself. BTW ergot is fucking absolutely disgusting to work with and should be avoided at all costs, as it promotes gang green! Would you really like to see a limb rot and have to be amputated all because you wanted a few doses of crapy LSD? Please take a chemistry course
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Look on the lycaeum, its there in some detail
"trip not equal to tryptamine, tryptamine equals trip"
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Thanks Zephler you were a real help!
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Foxy,
OK, I know that it grows in rye (also oats/wheat/etc - is it true that it will grow in a bowl of whole wheat cereal if I wet it and let it sit?), but does anyone know what conditions are required to cause the infestation to start?
Also does this affect cereals in OZ?(If so there must be spores in the atmosphere, all over the world eh?)
Also can I produce Lysergic acid by Alkaline Hydrolysis of the various alkaloids (anti Migraine / Parkinsonian treatments), I would appreciate further information on either, also on whether or not it works with LSA (with details please)
Yours
ALice D25
"Trust in God, but tie your Camel" [Arab Proverb]
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a bowl of cereal? god knows what the fuck will sprout in there (botulism)! try roquefort cheese (and maybe other blue cheeses). take a look into mycelia(mushroom) culture on agar. i may be able to dig something up on aspergillis culture too, let us konw if you want it(it's a little less toxic than ergot). :) ergot seems to grow on just about any wild grass, under wet conditions.
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I didn't mean to be rude man, I'm just trying to warn you against playing with fire, cause you will get burned. If you really want to know all about ergot search on the net, there is so much stuff out there on it and LSA your eyes will roll. But really ergot poisoning is very serious, limbs turn black and rot off, seriously. It was called St. Anthony's fire in the late 16th century (ergot posioning that is), today its called gan grene (spelling?). Anyways you can collect a few clumps of ergot from a soggy rye feild and then innoculate agar dishes with it and yes cultivate ergot, but the conditions for success are very hard to achieve (much harder than say P. Cubensis cultivation) and really out of scope of most people. If you want a good natural source of LSA, try Morning Glory Seeds, or Hawian Baby Woodrose seeds, both have LSA in them. Sure other unwanted molecules will likely follow through the synth, but this isn't analytical chemistry right? Anyways, the real way that anyone with a good chemical background approach the problem is to simply build LSA with something we call a Grignard reagent (RMgX), which adds carbons to a chain. Its like building something out of LEGO, just assemble the small pieces and presto! You have a dinosaur! If you ever do get a chance, research chemistry, it really is a beautiful thing! And please research, I don;t like to hear in the news about people getting hurt making dope!
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Well since you asked, but don't say I didn't warn you....
- Michael Valentine Smith: Psychedelic Chemistry
From pages 105-107:
The Culture and Extraction of Ergot Alkaloids
Make up a culture medium by combining the following
ingredients in about 500 milliliters of distilled water
in a 2 liter, small-neck flask:
Sucrose .................................... 100 grams
Chick pea meal .............................. 50 grams
Calcium nitrate .............................. 1 gram
Monopotassium phosphate ...................... 0.25 grams
Magnesium sulphate ........................... 0.25 grams
Potassium chloride ........................... 0.125 grams
Ferrous sulphate heptahydrate ................ 8.34 milligrams
Zinc sulphate heptahydrate ................... 3.44 milligrams
Add water to make up one liter, adjust pH 4 with ammonia
solution and citric acid. Sterile by autoclaving.
Inoculate the sterilized medium with Claviceps purpurea
under sterile conditions, stopper with sterilized cotton
and incubate for two weeks periodically testing and
maintaining pH 4. After two weeks a surface culture will
be seen on the medium. Large-scale production of the fungus
can now begin.
Obtain several ordinary 1 gallon jugs. Place a two-hole
stopper in the necks of the jugs. Fit a short (6 inch)
glass tube in one hole, leaving 2 inches above the stopper.
Fit a short rubber tube to this. Fill a small (500
milliliter) Erlenmeyer flask with a dilute solution of
sodium hypochlorite, and extend a glass tube from the rubber
tube so the end is immersed in the hypochlorite. Fit a long,
glass tube in the other stopper hole. It must reach near
the bottom of the jug and have about two inches showing
above the stopper. Attach a rubber tube to the glass tube as
short or as long as desired, and fit a short glass tube to
the end of the rubber tube. Fill a large, glass tube (1 inch
x 6 inches) with sterile cotton and fit 1-hole stoppers in
the ends. Fit the small, glass tube in end of the rubber
tube into 1 stopper of the large tube. Fit another small
glass tube in the other stopper. A rubber tube is connected
to this and attached to a small air pump obtained from a
tropical fish supply store. You now have a set-up for pumping
air from the pump, through the cotton filter, down the long
glass tube in the jug, through the solution to the air
space in the top of the jug, through the short glass tube,
down to the bottom of the Erlenmeyer flask and up through
the sodium hypochlorite solution into the atmosphere. With
this aeration equipment you can assure a supply of clean air
to the Claviceps purpurea fungus while maintaining a sterile
atmosphere inside the solution.
Dismantle the aerators. Place all the glass tubes, rubber
tubes, stoppers and cotton in a paper bag, seal tight with
wire staples and sterilize in an autoclave.
Fill the 1-gallon jugs 2/3 to 3/4 full with the culture
medium and autoclave.
While these things are being sterilized, homogenize in a
blender the culture already obtained and use it to inoculate
the media in the gallon jugs. The blender must be sterile.
Everything must be sterile.
Assemble the aerators. Start the pumps. A slow bubbling
in each jug will provide enough oxygen to the cultures. A
single pump can, of course, be connected to several filters.
Let everything sit a room temperature (25 C) in a fairly
dark place (never expose ergot alkaloids to bright light -
they decompose) for a period of ten days.
After ten days adjust the culture to 1% ethanol using 95%
ethanol under sterile conditions. Maintain growth for another
two weeks.
After total of 24 days growth period the culture should
be considered mature. Make the culture acidic with tartaric
acid and homogenize in a blender for one hour.
Adjust to pH 9 with ammonium hydroxide and extract with
benzene or chloroform/iso-butanol mixture.
Extract again with alcoholic tartaric acid and evaporate
in a vacuum to dryness. The dry material in the salt (i.e.,
the tartaric acid salt, the tartrate) of the ergot alkaloids,
and is stored in this form because the free basic material is
too unstable and decomposes readily in the presence of light,
heat, moisture and air.
To recover the free base for extraction of the amide of
synthesis to LSD, make the tartrate basic with ammonia to pH
9, extract with chloroform and evaporate in vacuo.
If no source of pure Claviceps purpurea fungus can be
found, it may be necessary to make a field trip to obtain
the ergot growths from rye or other cereal grasses. Rye
grass is by far the best choice. The ergot will appear as a
blackish growth on the tops of the rye where the seeds are
and are referred to as "heads of ergot." From these heads
of ergot sprout the Claviceps purpurea fungi. They have
long steams with bulbous heads when seen under a strong
glass or microscope. It is these that must be removed
from the ergot, free from contamination, and used to
inoculate the culture media. The need for absolute sterility
cannot be overstressed. Consult any elementary text on
bacteriology for the correct equipment and procedures.
Avoid prolonged contact with ergot compounds, as they are
poisonous and can be fatal. ----------------
The whole part with the pump is unecessary, you can get
micropore 1-gallon jugs from www.fungi.com, and alot of
the gear you would need, obtaining a pure strain sounds
like the tricky part, culturing and selection of
pure-looking samples a couple times should do it.. LSD
must be synthesized, it's such a beautiful molecule...
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ok, so any help on a grignard reagent to get to lsa? I just so happen to like my limbs the way they are but am intrigued.
the devil is so lonely.... >:(
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well look at the structure of LSA and see where you could make a good split down the middle producing, say an aldehyde and a alkyl chain or aromatic ring, that is readily avaliable in the alkyl halide form, I've once heard mentionings of bromocyptine...
.....to see in the workings of man, take a chemistry class
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Thanks for the details on the culture of the ergot fungus, I take your point on ergot poisoning, but heh, I'll either be classeda s witch or a saint, same as in the olden days!!
Yours
Alice D25
"Do the St Vitas Dance!"
"Trust in God, but tie your Camel" [Arab Proverb]