Author Topic: Quantitation of Psilocin in Human Plasma by HPLC  (Read 6291 times)

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Quantitation of Psilocin in Human Plasma by HPLC
« on: August 11, 2004, 06:24:00 PM »
I apologize for starting a new thread on this subject, this is my first post in the tryptamine forum AFAIK, thought it would be useful to those that hang out here.  Mod's feel free to move this to a more relevant thread.

Quantitation of psilocin in human plasma by high-performance
liquid chromatography and electrochemical detection: comparison of liquid–liquid extraction with automated on-line solid-phase extraction

Hiltrud Lindenblatt, Edda Kramer, Petra Holzmann-Erens, Euphrosyne Gouzoulisb-Mayfrank , Karl-Artur Kovar
Journal of Chromatography B, 709 (1998) 255–263


Two modifications of the HPLC–ED method with respect to extraction procedure used have been developed for psilocin, the active metabolite of psilocybin, in human plasma using either liquid–liquid extraction (LLE) or automated on-line
solid-phase extraction (on-line SPE). Each type of the sample preparation required a different HPLC system followed by electrochemical detection at 650 to 675 mV. The limit of quantitation of both modifications was 10 ng/ml psilocin. There was no significant difference observable between the LLE and the on-line SPE in terms of method standard deviation (LLE 1.82%, on-line SPE 1.13%) and the analytical results. However, the advantages of on-line SPE in addition to different selectivity were less manual effort, smaller plasma volumes of 400 ml (LLE 2 ml) and a recovery of psilocin in human plasma of nearly 100% (LLE 88%). In contrast to a previous procedure both methods were rapid, simple and reliable and yielded high plasma recoveries. They were used successfully in the quantitation of psilocin in plasma samples obtained from healthy volunteers after p.o. administration of 0.2 mg psilocybin per kg body mass. Plasma concentration curves and pharmacokinetic parameters were calculated.