Can the spore/H2O solution from the bought syringe be applied directly to dry agar in a petri dish to grow Mycellia. would it be nescsary to provide some sort of surface or other nutrients for the Mycellia to grow, Perhaps some liquid dextrose or does the agar provide all the nutrients nescesary. How moist should one keep the Culture of mycelia to promote vigorous growth while at the same time minimizing contamination.The usual distributers of the Spore syrenges claim that their syrenges will innoculate up to a dozen jars with 4 sites per jar. Does this mean that 40 agar dishes could be produced from each syrenge using 1/4 cc per dish. or should each dish be innoculated with 1 full cc. Since the medium is agar then swim thinks that 1/4 cc will be sufficent.
On second thought
If 25ml of PDA/dish prepared according to
PDA ** Potato Dextrose Agar
Autoclave 200 grams of sliced potato in 1000 mL of tap water. Filter through a hankerchief. Make potato salad for the family out of the potato residue. To the turbid filtrate add 20 grams of glucose and 10 grams of agar. Add water to make 1 liter total volume. Usually used as slants. PDA slants give good growth of most fungi, Agrobacterium, Nocardia, and many others.
Perhaps small, autoclaved scotchbrite squares would provide a good surface for the Mycellia to grow on. and the 1/4 cc be applied to the scotchbrite square. Just brainstormin out load any comments.
VL_
