See the references in https://www.thevespiary.org/rhodium/Rhodium/chemistry/lsd.synthesis.txt (https://www.thevespiary.org/rhodium/Rhodium/chemistry/lsd.synthesis.txt)
- and try our fine search engine! It is in great shape, and as good as new - it is almost never used by people wanting to find information!
You might start my looking at the links in this post: Post 394021 (https://www.thevespiary.org/talk/index.php?topic=13152.msg39402100#msg39402100)
(El_Zorro: "From Dallis grass to lysergic acid (in theory)", Tryptamine Chemistry).
i can oder the c. paspali strain here http://www.dsmz.de/species/sp300279.htm (http://www.dsmz.de/species/sp300279.htm)
but i can choose out of 4 different strains can you tel me witch one is the best for paspalic acid production
also how do you get paspalic acid from the strains
i read somewhere that the fungi produces paspalic acid in the medium and that i can extract it from the medium using the a/b methode but i don`t know what that methode is can some one tel me of this extration is done also dos the fungi alway produce the acid or only under certen conditions if so wat are these condistions
Bubbleplate i am very interrestid i the liquid culture medium that is used to grow the fungi so that it produces high yields of paspalic acid
thnx and greetz ben
would produce. The discovery, or more accurately, the "creation" of the original Claviceps paspali strain F-550, that DID produce Lysergic compounds was not an easy task. Apparently, ONLY Claviceps strains that form sclerotia in plants, and "Sclerotia like" formations in culture (Tonolo calls them "pseudoparenchyma" in surface agar culture, and "synnemata" [singular] in submerged culture) will produce ANY Lysergic A. compounds. They used the the method of infecting/growing out rye seeds just to find suitable strains to start the process. Then they used the time honored techniques of strain isolation/testing and mutation to develop ever more "potent" strains. The UV (black) light they used to mutate the strains is NOT the type one uses to light up their Day-Glo posters, but rather the type that is found in germicidal lamps, and some PROM erasers:
http://student.ccbc.cc.md.us/biotutorials/protsyn/mutate.html (http://student.ccbc.cc.md.us/biotutorials/protsyn/mutate.html)
http://plato.acadiau.ca/courses/biol/Microbiology/mutation.htm (http://plato.acadiau.ca/courses/biol/Microbiology/mutation.htm)
Keep in mind that UV light is not the only thing that can induce mutations in living organisms. Chemicals, heat, cold, and other radiation like X-Rays, and Beta and Gamma rays can be used. One technique is to expose fungi to levels of the mutagen that kills 99.99% of them. The .01% that survive have a mutation that hopefully not only helped it survive, but just MIGHT also be linked to more Lysergic production. There are other techniques and there are whole books on the subject.
Could one get lucky and just happen to stumble on a wild strain that also happens to grow well in culture AND produce good quantities of Lysergic Acid compounds? Possible, but doubtful. More likely is that you'd have to follow the same steps Tonolo et al did and patiently isolate, grow, test, and mutate many wild strains before finding a "good" one.
A better path would be to get hold of a known L.A. producing strain and take it from there. You'd get decent yields and you could then do your own mutation experiments to try and grow a "super strain".