The Vespiary

The Hive => Chemicals & Equipment => Topic started by: Plague on May 24, 2003, 08:20:00 AM

Title: Spectrophotofluorometer of any use?
Post by: Plague on May 24, 2003, 08:20:00 AM

Simple enough question, research has proven interesting, but would like to hear from those who know better...
Would a spectrophotofluorometer be of any use for analysis?

Title: i don't know of how much use...
Post by: blaaky on May 24, 2003, 09:44:00 PM

All the spectrophotometer can tell you is the concentration of a colored species in liquid... I mean we did some quantitative analysis with it in a chem lab. Some ion forms a colored complex, then do dilutions and a few simple calculations and you have the molarity of the ion. *shrug*

-blaaky

Title: Actually, if I understand ...
Post by: SpicyBrown on May 25, 2003, 11:35:00 AM

Actually, if I understand "spectrophotofluorometer" correctly.. The device in question is a fluorescence soectrometer, which does not tell you the concentration of a colored species in solution. A fluorescence spectrophotometer measures emission, not absorption/transmittance.  Such a thing probably is not of a whole lot of use for like, determining the identity of an unknown substance, unless the substance you're looking for fluoresces, and you know what its emission spectrum looks like..

-SpicyBrown