Author Topic: Spectrophotofluorometer of any use?  (Read 1286 times)

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Plague

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Spectrophotofluorometer of any use?
« on: May 24, 2003, 08:20:00 AM »
Simple enough question, research has proven interesting, but would like to hear from those who know better...
Would a spectrophotofluorometer be of any use for analysis?

blaaky

  • Guest
i don't know of how much use...
« Reply #1 on: May 24, 2003, 09:44:00 PM »
All the spectrophotometer can tell you is the concentration of a colored species in liquid... I mean we did some quantitative analysis with it in a chem lab. Some ion forms a colored complex, then do dilutions and a few simple calculations and you have the molarity of the ion. *shrug*

-blaaky


SpicyBrown

  • Guest
Actually, if I understand ...
« Reply #2 on: May 25, 2003, 11:35:00 AM »
Actually, if I understand "spectrophotofluorometer" correctly.. The device in question is a fluorescence soectrometer, which does not tell you the concentration of a colored species in solution. A fluorescence spectrophotometer measures emission, not absorption/transmittance.  Such a thing probably is not of a whole lot of use for like, determining the identity of an unknown substance, unless the substance you're looking for fluoresces, and you know what its emission spectrum looks like..

-SpicyBrown