the alkaloids are commertially available, somewhere
can't name sources so you'll have to find it on your own
The methoxy is right where the 4-hydroxy is on psilocin, now ya know.
the other name Mitragynine,
the longggg name Indolo[2,3-a]quinolizine-2-acetic acid, 3-ethyl-1,2,3,4,6,7,12,12b-octahydro-8-methoxy-a-(methoxymethylene)-, methyl ester, (aE,2S,3S,12bS)
CAS RN: 4098-40-2
Here is an article for you to check out
Structure revision of mitragynaline, an indole alkaloid in Mitragyna speciosa
Tetrahedron Letters (2001), 42(9), Pgs 1741-1743
Hiromitsu Takayama, Hayato Ishikawa, Mika Kurihara, Mariko Kitajima, Shin-ichiro Sakai, Norio Aimi, Hiroko Seki, Kentaro Yamaguchi, Ikram M. Said and Peter J. Houghton
Since You wanted to know, I dug up an extraction procedure.
Alkaloids from Mitragyna speciosa
Phytochemistry, Volume 30, Issue 1, 1991, Pages 347-350
Peter J. Houghton, Aishah Latiff and Ikram M. Said
Abstract
The major alkaloids present in very young leaves of Mitragyna speciosa from Malaysia were shown to be highly conjugated indoles, mitragynaline and corynantheidaline. Related compounds are also present in smaller amounts and two others were isolated and characterized (named mitragynalinic acid and corynantheidalinic acid). Mitragynaline is also present as a minor component in mature leaves of the plant.
Excerpt:
Isolation and extraction of mitragynaline(1). Dried, very young
leaves (135 g) were collected from trees of Mitrugyna speciosu
Korth. growing on the campus of Universiti Kebangsaan Mal-
aysia. The material was authenticated at source and a specimen
voucher is deposited in the herbarium of the Botany Depart-
ment, Universiti Kebangsaan Malaysia. The leaves were ex-
tracted with cold MeOH for 3 days and the extract filtered off.
After concn under red. pres. the extract was treated with 10%
HOAc and washed with 2 x 50 ml petrol (bp 60-80C). The aq.
acid layer was made alkaline with Na2CO3, and extracted with 3
x 30 ml CHCl3,. The CHCl3, layers were combined, washed with
H2O and concd under red. pres. to yield 3.24 g of a dark brown
residue.
TLC examination of this residue showed the presence of
several yellow zones. Prep. TLC was used (Merck Kieselgel
PF,254: CHCl3,-EtOAc, 4:l) to separate five bands IKl-IK5
with a yellow or orange colour in daylight and giving fluore-
scence under UV light 365 nm. Yields after elution from the silica
were low and decomposition appeared to be taking place.
However a sufficient yield of IKl(70 mg) and IK5 (55 mg) were
obtained for a full range of spectra to be performed. IKl and IK5
were seen to be mixts of two compounds each from the spectra
obtained and after some difficulty the mixts were finally separ-
ated using RP-18 silica gel TLC plates (Merck) with
MeOH-H2O (4: 1) as the mobile phase. Prep. TLC using this
system resulted in the isolation of the two separate components
in each case which were designated IKlA (30 mg), IKlB (25 mg),
IK5A(20 mg) and IK5B (16 mg) and named mitragynaline, cor-
yn~theidaiin~ mitragynalinic acid and coryn~th~dinalic acid
respectively.
Amounts of IK2, IK3 and IK4 were sufficient only for UV and
MS (2 mg each).
TLC examination of an alcoholic extract of 500g mature
leaves of M. speciosa obtained from Perlis State (voucher
specimen deposited in the herbarium of the Department of
Pharmacy, Universiti Sains Malaysia, Penang) showed the pre-
sence of IKl in small amounts. The concd extract (50 g) was
dissolved in HOAc, diluted with H2O and after standing over-
night the mixt. was filtered. The filtrate was made alkaline with
Na2CO3 and extracted with 3 x 50 ml CHCl3,. The organic layer
was taken to dryness under red. pres. to yield 1.8 g residue. The
residue was dissolved in CHCl3, and treated to vacuum liquid CC
using TLC grade silica gel (Merck Kieselgel G) 5 x 1 cm eluting
with CNCl3, (15 x 25 ml) and CHCl3,-MeOH, 19: 1(10x 25 ml).
The eluates were monitored by TLC and IKl was detected in the
latter eluates. Mitragynine was also present so the two were
separated from the coned bulked frs by prep TLC (Merck
Kieselgel PF, 254, CHCl3,-Et20 (4: 1) and eluted with (Me2CO) to
give mitragynine (5) 55 mg and IKI (1) 30 mg after recrystalliz-
ation from dry EtOH. Analytical TLC on RP-18 SG plates
showed no trace of IKlB in the IKl and so it was presumed that
only mitragynaline IKlA was present; this was confirmed when
spectra were run. Mitragynine was identified by spectral and
TLC comparison with literature values and samples previously
isolated in the laboratory at King's College. NMR spectral
features for both compounds were identified by 2D COSY,
NOESY and 'H-13C ~rrelation spectra.
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