The Vespiary

The Hive => Methods Discourse => Topic started by: PolytheneSam on May 26, 2002, 06:37:00 AM

Title: TLC silica gel IB2-F
Post by: PolytheneSam on May 26, 2002, 06:37:00 AM

Are TLC plates typically given a rating of some kind?  If someone had something like Baker-flex silica gel IB2-F (1B2-F?) what does that refer to?  They're 2.5 x 7.5 cm.

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http://www.geocities.com/dritte123/PSPF.html
The hardest thing to explain is the obvious

Title: the f means flurocent (they glow in uv).
Post by: hest on May 26, 2002, 07:53:00 AM

the f means flurocent (they glow in uv). The usual TLC plate made of an .1-.25mm silicagel 60 layer with a flurocent additive.

Title: fluorescent
Post by: PolytheneSam on May 26, 2002, 02:58:00 PM

OK.  Would something that is normally fluorescent show up on these plates in either short wave (hand held mineral light) or long wave (fluorescent black light), ie. something with a benzene ring or indole ring?

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http://www.geocities.com/dritte123/PSPF.html
The hardest thing to explain is the obvious

Title: Will be visible.
Post by: terbium on May 26, 2002, 03:51:00 PM

The organics will show up one way or another. Often the aromatic group will absorb the UV giving a dark spot but if the organics fluoresce themselves then it will likely be a different color from the green fluorescence of the TLC plate so they will still show up. Another quick way to visualize the organics on a TLC plate is to expose the plate to iodine vapour, the organic will absorb the iodine vapour causing a visible dark spot.