High Bees!
Swin got a 2nd hand analytical HPLC into her hands. The apparatus was set up (Pump -> Rhedyne 6way-valve -> spherisorb column -> UV/VIS detector) and connected to a PC via an serial Analog/Digital-Converter...
Distilled water is used as solvent for the first test runs, but will be replaced by MeOH or MeOH/H2O during the next weeks...
So i started the first trial with 1,3ml/min flow rate - a pressure of 2500psi build up, and some extremely diluted ascorbic acid solution was injected. - The result was a very nice peak at a retention time of about 5 minutes. (detection @ 270nm).
The second trial was made with a Ascorbit acid/acetyl salicylic acid mixture. - The ASA-Peak came 2 minutes after the vitamine c peak.
Swim tried also to inject solutions of
- 2C-B.HCl
- TCP.HCl
- PCP.HCl
- PCM.HCl
, but none of these substances caused a peak at 270nm.
Now my questions - are our favorite amine´s hydrochlorides not detectable with UV, or do i use the false wavelenght?
What column would be the best for our purposes?
Does anybee have some general tips regarding HPLC-Analysis? SWIm is primary an organic bee
, but the analytic stuff seems interesting too
Have a nice weekend!
xicori