Lol, well lets say I wish him luck and no Saint Anthony's Fire!!!
Here are some articles
Production of peptide ergot alkaloids in submerged culture by three isolates of Claviceps purpurea.
Amici et al. 1969-464 Amici, A. M.; Minghetti, A.; Scotti, T.; Spalla, C.; Tognoli, L. (1969)
Appl Microbiol 18(3):464-468
On an alkaloid from ergot.
Anonymous (1935)
Science 82(2114):16
A note on the variability of alkaloid content of domestic ergot.
Atal, C. K.; Schwarting, A. E. (1957)
J Am Pharmaceut Assoc Sci Ed 46:451
The new ergot alkaloid.
Dale, H. H. (1935) Science 82(2118):99-101
Effect of autoclaving and conventional and microwave baking on the ergot alkaloid and chlorogenic acid contents of morning glory (Ipomoea tricolor Cav. cv.) heavenly blue seeds
Friedman, M.; Dao, L. (1990) J Agr Food Chem 38(3):805-808
Ergot alkaloid and chlorogenic acid content in different varieties of morning glory Ipomoea-spp seeds.
Friedman, M.; Dao, L.; Gumbmann, M. R. (1989) J Agr Food Chem 37:708-712
Recent advances in ergot pharmacology. Phillipson
JD & Zenk MH (ed) Indole & biogenetically related alkaloids.
Academic Press, London :285-291
Fluckiger, E. (1980)
Studies on cultural requirements of Claviceps purpurea and inactivation of ergotamine.
Michener, H. D.; Snell, N. (1950)
Am J Bot 37(Jan):52-59
Title: Biosynthesis of Ergot Alkaloids by Immobilized Mycelium of Claviceps paspali
Authors: Matošiæ, Sreæko; Mehak, Milena; Ercegoviæ, Lidija; Okwor, James
Journal: Prehrambeno-tehnol. biotehnol. rev
Number: 2
ISSN: 0352-9193
Volume:30, pgs83-88 (1992)
Language: hrvatski
Summary: The effect of the selected parameters on the biosynthesis ofergot alkaloids during semicontinuous cultivation of immobilizedmycelium of Claviceps paspali F-2057 was investigated. Comparingthe effect of various substrate mass in the me of biosynthesis ofergot alkaloids medium, it was established that the mediumcontaining 6% mannitol and 5% ammonium succinate yields moreergot alkaloids. For the biosynthesis of ergot alkaloids, 4.25g/L 6 days old mycelial inoculum appeared to be most favorable.Investigation of the effect of alginic acid concentration usedfor the immobilization, showed 4% alginic acid concentration tobe the mest favorable for alkaloid biosynthesis. Insemicontinuous process, the immobilized Claviceps paspalimycelium produced alkaloids over a period of 60 days (6reincubations). Productivity of the process in each of thereincubations with immobilized cells is 75 to 120 % higher thanin corresponding free cell cultivations and the totalproductivity with immobilized cells is two times higher than thatof free cells. The results obtained here show that immobilizationof mycelia forming microorganisms, might be effective inbiotechnological production of complex metabolites, such as ergotalkaloids.
Title: Effect of antifoams on the biosinthesis of ergot alkaloids by high-productive strain of Claviceps paspali F-2057
Authors: Matošiæ, Sreæko; Mehak, Milena; Šuškoviæ, Jagoda; Golob, Zoran
Journal: Acta Bot. Croat.
ISSN: 0365-0588; Volume:53, pgs.39-47 (1994)
Language: engleski
Summary: The effect of several surfactants i.e. commercial antifoams on biosynthesis of ergot alkaloids was studied. Addition of some surfactants of polyglycole structure and Tweens to submerged cultures of high-productive strain of C. paspali F-2057 caused a change in alkaloid synthesis intesity. Pluronik (polyetoxypolypropoxy polymer) added in the range of 0.25 to 0.75 % markedly stimulated the production of ergot alkaloids. The Pluronik-supplemented culture reached maximal alkaloid yields one or two days erlier than the control. Production of alkaloids increased twice. The maximal yield achieved was 5.35 g/L with the process productivity amounting to 17.2 mg/L h.
Title: Physiological diferentiation of alkaloid producing strains of Claviceps purpurea (Fr.) TUL.
Authors: Matošiæ, Sreæko; Mehak, Milena; Šuškoviæ, Jagoda
Journal: Acta Bot. Croat.
ISSN: 0365-0588
53, pgs.21-30 (1994)
Summary: Four different strains of Claviceps purpurea IC/39/20-B, G, R and W have been selected and isolated using common selection method. The relationship between intensity of pigmentation of the culture and the accumulation of ergot alkaloids have been noticed. Maximal alkaloid yields have been obtained with more pigmented strains R and W in the sucrose-asparagine medium (1.30 and 1.50 g/L respectively) and with less pigmented strains B and G in the sucrose-peptone medium (0.30 and 0.80 g/L).
Title(Thesis): Biosynthesis of Ergot Alkaloids by means of Immobiliyed mycelium of Claviceps paspali
Summary: Comparing the effect of various substrate concentration on thebiosynthesis of ergot alkaloid, it was obtained that a mediumcontaining 6% manitol and 5% ammonium succinate yields more ergotalkaloid. For the biosynthesis of ergot alkaloid, 4.25 g/L dayold mycelial inoculum appeared most favorable. Investigating theeffect of alginic acid concentration used for immobilization,obtained results showed 4% alginic acid to be most favorable foralkaloid biosynthesis. In semicontinuous process the immobilized,Clavicepd paspali mycelium produced alkaloids over a period of 60days (6 reincubations). Dilution of basic medium, or reducing thesubstrate concentration reduces alkaloid yields almost in aproportional manner. However, basic media diluted in the ratio of1:10 gave in a relative ratio higher alkaloid yield. Productivityof the process in each of the reincubations with immobilizedcells is 35% and 50% higher then in corresponding free cellcultivations in all tested substrate media, and totalproductivity with immobilized cells is three times higher than offree cells. Effect of surfactant-pluronik (0.25%) on ergotalkaloid biosynthesis in the medium diluted in the ratioo of 1:4was tested and obtained results showed that alkaloid yieldincreased by about 35%. The results obtained here show thatimmobilization technique, also of mycelia forming microorganisms,might be effecttive in biotechnological production of complexmetabilites such as ergot alkaloids.
Do Your Part To Win The War