Arrogant japanese lab monkey (AJLaM) in a parallel universe
decided to abandon synthesis of DMT from Trp, due
to numerous failures.
Instead, she ordered samples of MHRB from various sources
and decided to follow A/B extraction:
[100g of MHRB powder -> HCL, pH 1.1 - three extractions, 3 days,
occasional (~1 hr) 60 C heat; filtered through cotton, then coarse glass/sand and then N1 paper filter; volume reduced to 300 mL under vacuum and ~80 C); when cooled, became cloudy - filtered through 1N filter again; removed fats with hexanes x2 times - some minor emulsions; basified with solid NaOH while monitoring pH. At pH ~6 the mixture turned grey. At pH ~11.6 became dark-red/black/a little cloudy; distinct indole smell, stopped at pH 12.1. Added 6 g of KCl;
extracted with hot hexanes x3 times - took whole day due to a lot of bubbles/emulsions which took more than 2 hrs to resolve; removed most of the hexanes with rotor at 35 C - yellow colored hexanes solution ~20 mL, some
small crystals strart to fall out at room temperature. Placed into isopropanol/dry ice - white crystalls form rapidly, in radial-snow-flake patterns, solvent less yellow. white crystals. Crystals were collected - m.p.41- 43 C, remaining solvent was placed on a glass dish;allowed to evaporate yilding some slightly yellow crystals - m.p. 41-43]
MHRB, source 1 - 490 mg from 100 g
MHRB, source 2 - 600 mg from 100 g
MHRB, source 3 - 330 mg from 100 g
AJLaM smoked some, it was all right.
AJLaM needs a quick tip/link on how to convert it to oxalate with oxalic acid for injection
Also, AJLaM has a question, if its O.K. to prepare solution with lemon juice and inject that.
AJLaM would be more than thankful.
And even more thankful if no TFSE is mentioned:)