Electrophoresis separation

[ClearLight]

  I had not normally thought of this as a viable purification method, however I just picked up copy of CL Stong's Amateur Scientist book from the 70's and there on the Electroph. article is a discussion about a 30 inch vertical plate with the charge applied across the entire horizontal vector... The individual used it for rapid large scale purification, by catching the right, left and middle flows down the plate...

  This strikes me as a novel method for purification/separation that could be accomplished with say,  electrolytess, glass plate, fiberglass cloth and 200 volts D.C., without the vac distillation etc...

 Any thoughts?

Infinite Radiant Light - THKRA

[SPISSHAK]

Is this like chromatography assisted by electric current or something?

[foxy2]

Not applicable to large scales.
They are struggling to get up to gram scale seps.


Those who give up essential liberties for temporary safety deserve neither liberty nor safety

[ClearLight]

 so foxy, what's the problem with this in large scale?


Infinite Radiant Light - THKRA

[foxy2]

your applying a huge voltage, which requires very good cooling or you cook the stuff.  Thats why they normally do electrophoresis in a capilary tube, easy to keep cool.
Those who give up essential liberties for temporary safety deserve neither liberty nor safety

[hest]

Hmm might work for 100mg shitty acid. Make an gel plate (agar ?) ad the 100mg and aply power from the neontube (with a diode

[ClearLight]

Not to beat this to death, but it seems that the problem is that the substances to separate ( and what we are interested in) have weak charges, so the separation is not efficient...

  Does anyone think that might work??


  What would happen if you irradiated the area with laser light tuned to the orbital wavelength of what you were interested in???  Similar to the atomic vapor isotope separation???  Then you would have a selective charge on your molecule, that should, afaik, make it more mobile across the voltage differential?


Infinite Radiant Light - THKRA

[foxy2]

New capilary electrophoresis techniques have gotten separations of over a million theoretical plates.
Those who give up essential liberties for temporary safety deserve neither liberty nor safety

[dwarfer]

The migration is a function of molecular charge,
impressed voltage,
and media.

You can use 200 volts and watch it migrate,
or 24 volts and come back next week.(Exaggeration.)

A piece of blotter paper, between two glass plates,
with the overhanging ends immersed into the electrode
pools...

Hmm:  meth FB has almost zero charge: ephedrine does not have much.

Wonder...

Just put your 5 grams in the middle, and come back next week to collect your 2.3478 grams of pure shitola??

[foxy2]

Any free amine has lots of charge and should easily bee able to bee separated with electrophoresis, at very small scales.

[hest]

´The idea is fun, but I don't think it has any practical use. With 'standard' chromatography (dry colum ect.) you can seperate almost any thing in a few houers in the 10-100g scale.

[Lilienthal]

A 'freebase' amine doesn't carry a charge. If you want an amine to move in the electric field, you have to protonate it by adjusting the pH.