Author Topic: TLC Time  (Read 9207 times)

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ClearLight

  • Guest
TLC Time
« on: May 03, 2002, 04:12:00 AM »
After going through the search engine and rhod's site, I realized there was no easy faq or work up for TLC, even ghetto variety.

 What I'd like to request is that folks list their experience/research/cites for TLC workup.  Please post commentary elsewhere and we'll keep this on track. I'll assemble all of this into an easy to use guide for refinement and experimentation.  Here's the suggested format.

  Target Compound:

  Media: (paper/cellulose/silica/etc. )

  Elution Solvents: (EtAc,MeOH etc w/ ratios)

  Visualization: reageants if any or U.V.

  rF: for target compounds and any secondaries or unreacted
     secondary/precursor compounds



Infinite Radiant Light - THKRA

lugh

  • Guest
USTFSE!!!
« Reply #1 on: May 03, 2002, 11:55:00 AM »

After going through the search engine and rhod's site, I realized there was no easy faq or work up for TLC, even ghetto variety.



This is nonsense, see

https://www.thevespiary.org/rhodium/Rhodium/chemistry/psilo-tlc.txt

and

Post 108792 (missing)

(dormouse: "Lab Suggestions  -ymir", Novel Discourse)
topics covered are:

Use of Ethanol-Water Adsorbent Slurries in Coating of Thin-Layer Chromatographic Plates
An Inexpensive Thin-Layer Chromatographic Spreader
Devices for Thin-Layer Chromatography
Transfer Tool for Thin-Layer Chromatography
Technic and Enclosure for Rapid Chromatographic Spotting
Technic for Separation and Solution of Zones in Thin Layer Chromatography
Desiccation Box for Thin-Layer Chromatographic Plates

From equipment fabrication to simple how to's, it's in that post  :o  Further information not available on Rhod's page and that post would be welcome  :)





Sunlight

  • Guest
Little info.
« Reply #2 on: May 03, 2002, 05:53:00 PM »
There's litlle information about TLC in the Hive, Osmium and other bee (Ritter?) posted rf of iso, ketone and glycol with hexane:ethyl acetate and toluene:ethyl acetate system, that are very good for this purpose. Methanol:NH3 ,10:1 or so is also good for amines, but we have not an extensive work showing how to make TLC and some common rf of interesting products. Anyway the topic has been discussed, the use of UV plates and the appropiate lengthwave etc...

Rhodium

  • Guest
Zubrick TLC Chapter
« Reply #3 on: May 03, 2002, 06:14:00 PM »
Here is the TLC chapter from Zubrick, in DejaVu format:

https://www.thevespiary.org/rhodium/Rhodium/chemistry/equipment/tlc.zubrick5.djvu



You need the DejaVu browser plugin to be able to read it:

http://www.lizardtech.com/includes/download.php?p=1&o=1


ClearLight

  • Guest
UTFSE -----
« Reply #4 on: May 03, 2002, 07:17:00 PM »
No lugh, your full of shit... I have used the search engine, if you had bothered to do it, you would see that this is all that there is...Ymir's stuff doesn't resolve with a "TLC" search, and it's nice that he copied all of that stuff right out of the same book I have, but if it was so fucking great and easy to use, then why isn't it happening... because it's TOO obtuse... you have to downsize it for less tech minds, that is unless your happy over in Methods explaining for the umpteenth time why the benzowacker didn't work and folks can't tell what intermediates they got because they did even know how to do simple TLC!

<----------- SEARCH ENGINE RESULTS TLC ------------------------------------->

Isosafrole
TLC plate was silica gel, mobile phase 97:3 toluene/ethyl acetate.

anethole
GC of the anise oil showed three peaks. one of these is about 90area% of all peaks. otto assumes that this is anethole. on TLC there was only one spot visible under UV-
light as well as with permanganate (RF 0.8 - 0.9 with Hexane/EtOAc 4:1).

(11) anethole is very active in UV. TLC is thus a good proof of proper separation.
RF: anethole 0.8 - 0.9, PMP2P 0.3 in Hexane/EtOAc 4:1.


That Pd(OAc)2 / tBuOH-H202 Wacker does not produce MDP2P with safrole. It produces a damned near perfect yield of the aldehyde which is useless for our purposes. This was proved by TLC. The sample was tested against safrole, isosafrole, 3,4MD propiophenone and MDP2P on whatman MK6F silicagel plates using 97:3 toluene/ethyl acetate mobile phase. The rf of the product produced by that oxidation was different than all of the mentioned compounds and also aminated w/ MeAM perfectly leaving me to conclude it was the aldehyde.

IR absorption information



,4,5-Trimethoxybenzaldehyde Synthesis
by Hest

5-Bromovanillin
Add 15,2g vanilin and 17,6g Br2 to 75ml AcOH, let the solution stir 1 h. Pour out the solution in 250mL ice-water. filter off the product. Dry and recrystallise from ethanol. mp. 162?C. TLC Rf 0,47 EtOAC:P 1:1

TLC Rf 0,35 EtOAc:P 1:1

<---------------------------------END SEARCH ENGINE TLC ----------------------------------->

  That is hardly sufficient for a MDMA visualization wouldn't you agree??

  as to the psilocybin post on rhodiums site, it's very weak. I have the paper that gives 160 color points on every imaginable tryptamine using Erlich's, compared to a flower color scale...  Nothing like that yet for PEA's though...
 
  So before you go off like your flaming a newbee, take a look at exactly what I asked for. There is NOTHING in the hive that says anything about a visualization agent for MDMA for example all though most in the know realize that a Marquis test can do it for your ( dance safe etc. )  Is that anywhere here???

  And is there anything that tells people how to roll their own plates easily??? NO!  ( Nice wash info and spreading)
 
  So I think someone should dump some karma points on you and you should CHILL out...maybe your just having a BAD DAY...

  And then contribute something to this thread...  you know,  I really don't have to bother doing this if it is just a waste of time...


Infinite Radiant Light - THKRA

ClearLight

  • Guest
Zubrick
« Reply #5 on: May 03, 2002, 07:22:00 PM »
Thanks rhodium, that was exactly what I was considering writing up on the methods, now we just need the elutents and the visualization stuff...

btw a TLC search on your site, Thin-Layer Chromotography and Thin Layer Chromotography, only resolve to the psilo.txt file, not this info in zubrick...


Infinite Radiant Light - THKRA

Rhodium

  • Guest
Zubrick
« Reply #6 on: May 03, 2002, 10:50:00 PM »
That is because I scanned/uploaded this article today because you asked for TLC info, I haven't yet linked it from my main chemistry page.

lugh

  • Guest
Flame On
« Reply #7 on: May 04, 2002, 01:37:00 AM »

No lugh, your full of shit... I have used the search engine, if you had bothered to do it, you would see that this is all that there is...Ymir's stuff doesn't resolve with a "TLC" search, and it's nice that he copied all of that stuff right out of the same book I have, but if it was so fucking great and easy to use, then why isn't it happening... because it's TOO obtuse... you have to downsize it for less tech minds, that is unless your happy over in Methods explaining for the umpteenth time why the benzowacker didn't work and folks can't tell what intermediates they got because they did even know how to do simple TLC!




I guess using the search terms "thin", "layer" & "chromatography" is too advanced for some newbees :)  Would you care to share the title of that book that thread was copied from with us, or is that too much to ask?

As far as

Post 304549

(lugh: "USTFSE!!!", Methods Discourse);

Post 304428

(ClearLight: "TLC Time", Methods Discourse)
suggests that there is no information on the Hive already, which isn't the case  :)


And is there anything that tells people how to roll their own plates easily??? NO!  ( Nice wash info and spreading)
 
  So I think someone should dump some karma points on you and you should CHILL out...maybe your just having a BAD DAY...

  And then contribute something to this thread...  you know,  I really don't have to bother doing this if it is just a waste of time...




It appears you weren't aware of that thread (despite what was said earlier), so a contribution has already been made (albeit not one that floats your boat). As far as the bad karma points, flaming such as this is one way to get them, be glad you haven't received such  :)  


What I'd like to request is that folks list their experience/research/cites for TLC workup.  Please post commentary elsewhere and we'll keep this on track.




Post 108792 (missing)

(dormouse: "Lab Suggestions  -ymir", Novel Discourse) is the most thorough collection of TLC info currently on the Hive, thus fulfilling the requested criteria. As far as keeping the thread on track, there's no quicker way to go astray than to light up the flamethrowers  :(  


Understanding the language of science is necessary for advancement in this field  :)  

 


LaBTop

  • Guest
Ahumm,
« Reply #8 on: May 04, 2002, 11:04:00 AM »

Post 211877

(wirewound: "Distillation: How to Set Up a Distillation", Methods Discourse)
,
""In one of the links (

http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html

) I found these 2 TLC procedures at last:

http://orgchem.colorado.edu/hndbksupport/TLC/TLCprocedure.html


http://www.indiana.edu/~orgchem/movies/complt.mov


This is an animated graphics movie on TLC procedure. LT/""

That one I just did from memory. Nobody seems to read, and I mean really READ, (and click the links), my sticky threads in Newbee and Methods..

Then I used the FSE, and guess what I found using my brain, the provided instructions at the bottom of the Search page, and thus filling in:   "TLC"   observe the use of the "..." !!! :

Search results
Matches 1 to 25 of 480

So, who's full of shit here?  You've got a long way to go ClearLight!

Btw, I'm also curious what book you have with those Ymir articles in it, BECAUSE it doesn't come from a BOOK! It comes from a non published anymore, monthly magazine. LT/

PS: Why don't you copy and paste here that TLC info you have there at home (when you bought the sci-am Amateur Scientist CD Rom) f.ex. how to roll your own tlc plates out of microscope slides.

WISDOMwillWIN

ClearLight

  • Guest
Ok...
« Reply #9 on: May 04, 2002, 07:43:00 PM »
Lugh gets a formal apology, he is NOT full of shit. 

Post 281214 (missing)

(lugh: "Re: Thin Layer Chromatography", Chemistry Discourse)
is an excellent site with all the methodology explained...

re: Labtop Book.  The sci-am article was apparently taken from Zubrick and rhodium has done a great service and scanned it in on the link in the prior post.

  I believe this answers all the methodology issues...

  I did btw use the search engine and it did not pop up Lugh's prior post.  It did pop the 423 TLC references that lab top came up with prior to this thread, and going through that was how I got the prior listing... maybe I misspelled it, maybe I didn't quote it or I did quote it... anyway, I did do the search, but my results were not as good as others...

 

Infinite Radiant Light - THKRA

Sunlight

  • Guest
UV plates
« Reply #10 on: May 05, 2002, 12:46:00 AM »
If can buy commercial UV TLC silica gel plates and micropippetes, that's definitive the solution. You can find short 254 nm UV lamps that can be armed in a fake money decetor, not really expensive, it's what we are actually using, and it gives you the light you need in research.

ClearLight

  • Guest
All TLC question's now answered!
« Reply #11 on: May 06, 2002, 08:53:00 PM »
Your Tax Dollars at work...

  Here is a complete list of all various honey's, limits of detection, visualization reagant, reagent preparation, elutent/solute  and color scale values for returned color...

  

http://www.ncjrs.org/pdffiles1/nij/183258.pdf



 Enjoy!


( someone may wish to archive this in case it is made to disappear )

Infinite Radiant Light - THKRA

Rhodium

  • Guest
Color tests
« Reply #12 on: May 06, 2002, 09:06:00 PM »
Great! I archived it right away to

https://www.thevespiary.org/rhodium/Rhodium/pdf/colortestreference.pdf



13 minutes from ClearLight posting it to me having it on my page!

LaBTop

  • Guest
Ahumm,
« Reply #13 on: May 06, 2002, 11:44:00 PM »
I see very limited use for underground chemists for this, and they state that the chance of various false positives and negatives is quite big. It can in my humble opinion just give a VERY rough indication for very illiterate law enforcement officers to give them a reason to arrest an allready strange looking, acting or known abusive individual.
The real tests have to be done in a well equiped laboratorium, specialised on testing substances of abuse.
See for better tests:

Post 190939 (missing)

(LaBTop: "Re: Ecstasy testing kits", Chemistry Discourse)
Ecstasy testing kits  (Damm nearly the same reagents as these guys use, ain't it? This was already published in 1977!)

Post 189956 (missing)

(Chromic: "Ecstasy testing kits", Chemistry Discourse)
Better read the whole thread with the above post and the PATENT in it, and then the last post is very usefull for street vendors and cooks alike:

lunatic_asylum (Stranger) 08-27-01 05:19
No 207690
         Re: Ecstasy testing kits

Yes this should be the Simons Reagent!
There are three important reagents, which are very useful for testing pills.
1. Marquis-Reagent:
Into 50ml conz. H2SO4 there are dropped in, with ice cooling, 50 drops of formaline (35% in H2O!)

2. Simons-Reagent (this consists normally of three separate solutions):
1)  2% of Sodiumcarbonat in H2O
2)  4% sodium nitroprusside  in MeOH
3)  10% Acetaldehyde in MeOH
(Maybe in this EZ-test there are two of them mixed together)
You have to drop one single drop of the reagents (in the mentioned order! 1., then 2.) onto your substance.
A blue colour indicates a N-substituted compound like MDMA, MDE, Meth or PMMA

4. Gallic Reagent
0,5% Gallic acid in conz. H2SO4
This reagent is very interesting, because is shows you methylendioxygroups!!!
In the literature it should turn into blue/green (in my experience it turned into yellow and after one minute into yellow/green to green).
It reacts in this way with MDA, MDMA, MDE, MMDA, BDB and MBDB. With other substances there is no reaction!!!

I have worked with this three reagents many times and I think, in combination, they are very useful (and cheap!!!).


Post 123336 (missing)

(LaBTop: "Re: Seperating MDMA?", Newbee Forum)
Seperating MDMA?

Post 123318 (missing)

(EBOMB: "Seperating MDMA?", Newbee Forum)
For a good laugh, and some good info, read this whole thread, that's better. LT/

WISDOMwillWIN

ClearLight

  • Guest
yes...but...
« Reply #14 on: May 07, 2002, 01:04:00 AM »
Oh well, my motiviation on this was to develop visualization agents for the TLC analysis, so you have "SOME" idea of what it was, and of course, what reagents to start with... nice that they give you the formulations... none of it will ever = a GC/MS or NMR, but then I don't have one handy...

  I always had a fantasy about the ghetto variety quantitative spectrophotometer analysis, where you tlc'd the sample and the shot a digital image of the spot, that when compared to a reference in photoshop you could get some idea of how much you had... nice for plant sources at least..

 They do have your favorite formulations listed...3

 Looks like the same reagents as the old ex kits as well...although now dancesafe is marketing A10 mecke reagent that discriminates between dxm and x, gives a nice color for 2-ct-7 as well.

 Thanks for the gallic acid, didn't know about that one..


Infinite Radiant Light - THKRA

lugh

  • Guest
Characterization of Essential Oils
« Reply #15 on: May 21, 2002, 10:35:00 PM »
At alchemy_bee's request, from Analytical Chemistry 26 960-3 (1954)  :)

Prepare a mixture of 28.5 g of silicic acid, 1.5 g of corn starch, 0.0011 g of Rhodamine 6G, and 54 g of water. Stir the mixture while it is heating on a water bath at 85°. After the mixture thickens, remove it from the water bath and add 20 ml of water. Spread the mixture to give a layer 0.02 in. thick on glass plates 5 in. x 7 in. Dry the plates for 20 minutes in an oven at 105° and, then, for 30 minutes at a pressure of 2 mm over potassium hydroxide.
Apply 1 to 2 mg of each sample or mixture dissolved in a low-boiling hydrocarbon in small spots along a line 2 cm from a short edge of the plate. Place the plate (with the samples near the bottom edge) in a covered battery jar. The bottom of the jar should be covered well with a solution of 10 to 15% ethyl acetate in hexane. Allow the solvent front to move 12 to 14 cm from the initial line. Remove the plate from the battery jar and allow it to dry in the air.
Observe the plate under ultraviolet light, and draw with a pencil a ring about each spot found. Spray the plate with a solution of 0.4 g of 2,4 dinitrophenylhydrazine in 100 ml of 2N hydrochloric acid to detect carbonyl derivatives, and again mark the spots. Use ultraviolet light to locate traces of ketones. Place the plate in an oven at 105° for 10 minutes to cause heat- and acid-sensitive components to appear. Compare the locations and appearances of the spots with those caused by known compounds. If a permanent record is desired, trace the edges of the spots on a piece of transparent paper.


 

ClearLight

  • Guest
Cites - safrole TLC and Extraction
« Reply #16 on: May 28, 2002, 07:09:00 AM »
Don't have access to full text, maybe someone will post the articles...


from: J Chromatogr Sci 1994 Jul;32(7):253-8

SFE with GC and MS determination of safrole and related allylbenzenes in sassafras teas.

Heikes DL.

Total Diet Research Center, Food and Drug Administration, Lenexa, Kansas 66285-5905.

Safrole (4-allyl-1,2-methylenedioxybenzene), a natural plant component of the aromatic oil of sassafras root bark, possesses carcinogenic and mutagenic activity. Legal restrictions have been placed on safrole as a food additive. However, sassafras teas continue to be accessible from health food establishments in the United States. Supercritical fluid extraction (SFE) with gas chromatographic-mass spectrometric (GC-MS) determination is utilized in the formulation of a rapid, accurate, and specific method for the determination of safrole and related allylbenzenes in unbrewed sassafras teas. Samples are extracted in a static-dynamic mode with CO2 at 690 bar and 80 degrees C with methanol as an extractor-added modifier. Levels of safrole exceeding 10,000 mg/kg (1.0%) are commonly encountered. Lesser amounts of other allylbenzenes, including eugenol and 4-allyl-1,2-dimethoxybenzene, are also reported. Recoveries of safrole and related compounds from previously extracted tea samples fortified at 100 and 1000 mg/kg ranged from 96 to 101%.

 

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8063885&dopt=Abstract



      and...

J AOAC Int 1997 Sep-Oct;80(5):1023-8

Liquid chromatographic determination of safrole in sassafras-derived herbal products

Carlson M, Thompson RD.

U.S. Food and Drug Administration, Minneapolis, MN 55401, USA.

A liquid chromatographic (LC) method was developed for determining safrole in herbal products derived from sassafras (Sassafras albidum), as well as related compounds such as isosafrole and dihydrosafrole. The procedure involves solvent extraction and isolation of analyte by reversed-phase LC with UV detection at 235 nm. Safrole is resolved from related compounds and other sample constituents including thymol, a component of thyme. A linear concentration range of 0.003-0.200 mg/mL was obtained for safrole, isosafrole, and dihydrosafrole. Limits of detection (LOD) and quantitation (LOQ) were e0.0015 and 0.0051 micrograms/mL for safrole, 0.0018 and 0.0061 micrograms/mL for isosafrole, and 0.0038 and 0.0125 micrograms/mL for dihydrosafrole, respectively. Intraday relative standard deviations (RSDs) for safrole (n = 5) from various samples ranged from 1.30 to 5.39% at analyte levels of 0.01-1.5%. Safrole contents of 26 samples including root bark powder, leaves, oils, tea concentrate, herbal extract tinctures, and herbal powder capsules ranged from < LOD for most leaf samples to 92.4% for an oil. Recoveries of safrole from fortified samples ranged from 83.6% for an oil to 117.2% for a tincture preparation. Safrole contents of 0.09-4.66 mg/cup were found for brewed teas prepared from sassafras root bark powders and tinctures.

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=Display&DB=PubMed





Infinite Radiant Light - THKRA

ClearLight

  • Guest
The Van Urk-Salkowski Reagent - Indole Specific
« Reply #17 on: July 14, 2002, 04:25:00 AM »
Journal of Chromatography, 132(1977) 267-276

... A sensitive and specific chromogenic reagent for silica gel thin-layer chrmoatogrpahic detection and identification of indole derivatives

  Summary:  The chromogenic reagent described has been teseted with seventy-nine indole derivatives and found to be very sensitive and indole-specific. The lower limit of dectection on silica gel TLC was between 25 and 50 ng for most indoles.... The procedure was simple and required only 10 minutest from the time of spraying the TLC plate until full color development was reached.  The colors had a wide spectral range from the yellow of the indole-3-glyoxylamide chromophore to the blue of the melatonin chromophore and were extremely stable.


page 268 "... The Erlich and van Urk reagenst are, to date the most specific chrmogenic reagents for indole derivatives, but color development is slow ( 3-8h) and the colos are not stable due to mineral acid retained on the silica gel layer.  ... Color development with the salkowski reagent is rapid (15-30)min but the colors change quickly to non-diagnostic brown tones. ....

   A spray reagent has now been developed that has a high sensitivity and specificity for indole compounds, gives rapid color development and color stability of the indole condensation products."

pg 269:
    Solvents and Reagents:
    (A) Van Urk Reagent: 1 g p-dimethlyaminobenzaldehyde...dissolved in 50 ml Conc. HCL (s.g. 1.190) and 50ml Absolute ethanol was added. This reagent is stable for several months at room temperature when stored in a brown glass bottle.

   (B) Salkowski reagent (as modified by Tand and Bonner), 2.03g FeCl3*6H2O  dissolved in 500 ml H2O and 300 ml H2SO4 (s.g. 1.840).  This reagent is stable indefinitely.

   Spray Reagent:  The new TLC spray reagent used, was made up of Reagent A + Reagent B(1:3).  The spray reagent may be kept at room temperature for several weeks.

    TLC Solvent Systems:

    1) Butanone-ethyl actectate-ethanol-water (3:5:1:1)
    2) Propanol-Water(8:2)
    3) Propanol-water-28%NH4OH  (8:1:1)
    4) Chloroform-MeOH-H2O  ( 84:14:1)

 Methods:

    Visualization of indoles

    After 1 or 2 dimensional TLC of indole standards or extracts... the plate was dried at 45°C until all traces of solvent had evaporated. ... Spraying was done in a fume hood... until the silica gel layer became transparent...

  The plate was heated in a 100° oven for 5 minutes and allowed to cool to room temperature.  The place wsa immersed in distilled H2O... agitated periodically for 1 min.  (repeat 2x for non yellowing permanent gel record -CL)

  The plate was removed from the last water wash and blotted with a dry paper towel. At this time the colors of the indole condensation products were evaluated (table 1: wet-plate color reading). The plates were then dried at 45°C (20-30min). the colors of the indole condensation products were evaluated once more (table 1; dry-plate reading).  The colors of the indole condensation products are extremely stable and fade resistent. We have kept TLC plates at room temperature in the dark for more than two years wiht little of no faing of the original dry plate colors.

  ( partial list of colors )

 Compound                color region         color name

Indole-3-acetic acid     bluish violet     Aconite Blue(180)

Tryptamine               Blue              Princess Blue(98)

5-HTP                    Violet Blue       Sea Blue(119)

5-MEOTryptamine          blue              Princess Blue(98
                   (dry) Bluish Green      langite Green(53)


( unfortunately 5meo-dmt, nn-dmt are not included in this table, -CL)





Infinite Radiant Light - THKRA

ClearLight

  • Guest
Delivering the Goodies!
« Reply #18 on: July 15, 2002, 08:53:00 AM »

  So, after whipping up the above reagents, I tested it against 5HTP.  I put a microscopic amount on a watch glass, added 4 drops of reagent, and then heated slightly on the flame...within 30 sec's it colorized! Sucess!

  Anyway I got so inspired I pulled out my stash and did every indole I had... ( the dmt was left over from an extraction and failed to colorize...hmmm must still be in that emulsion >:( )

 I considered getting a color wheel and putting that up with the images, but how many of you have color wheels, so I grabbed the arm and hammer box for the red and yellow and the good ole' VM Naptha can for the blues... This should allow color correction for the purists, although the colors were different enough I wouldn't have any problem using them as they appear...
 
Anyway, for your viewing pleasure,  here are the images...



  This shows all of the tests in one shot...



  This shows the psilocybin, as a reddish brown and the 5meo-DIPT as a greenish blue... These test are really sensitive...



  Here is the lsd as a beautiful clear blue...this is 50 mics of pharmaceutical grade LSD-25 gc'd and mspec'd.

  The DPT or DipropyTryptamine is a much deeper slate/blue green...



  Here is the 5HTP, a cheap, legal way to test your solution, with another shot of the LSD-25



  Here is a reference shot of the 5meo-DMT.  It has a much more green tint to it, than the 5meo-DIPT, which has more blue... I may try another image if folks have any questions... and finally...



  In this shot, you can see the 5meoDMT having more green in the color and the 5meoDIPT having a bit more blue green...

  I hope this inspires folks to do more TLC, as you can see, it is a very useful and satisfying way to validate your results as you go through the experiments...

  One thing I would like to point out, is that if you have isolated your DMT, 5meo-DMT, or LSD... you can make your self a standard solution of your compound. Use this visualization reagent with your standard and your compound to test ( extract from your last psychotria order, mh root bark etc.) and by shooting a digital image and comparing the density of the standard against the unknown, you can get ( if your careful ) a fairly good indication of the amount of "active ingredient" in your unknown materials...

  Enjoy, and thx to all who make this possible!!!



Infinite Radiant Light - THKRA

Osmium

  • Guest
Nice! But it's still way too concentrated.
« Reply #19 on: July 15, 2002, 10:52:00 AM »
Nice!

But it's still way too concentrated. On a TLC plate you have much smaller amounts present, so the colours will be lighter. Same when doing testing of biological material. When the concentration is too high it all seems to turn dark blue eventually. Have you tried diluting the coloured samples with water?

I'm not fat just horizontally disproportionate.