The Hive > Tryptamine Chemistry
Psilocybin Production
paranoid:
While interesting, this is not exactly arcane material. It also is not especially relevant to the discussion at hand, in particular considering that the debate here is focussing on the level of alkaloid production in mycelial cultures of P. cubensis.
adroit_synth:
The PF cakes are colonizing now. :) :) :) Eighty-five is far superior to room temp as far as germination and colonization speed go, however with no quantitative test SWIM have nothing to say of the affect temperature has on alkaloid production (thinking maybe it is like shocking a cannabis plant, makes more cannabinoids). Looking into simple chromatography methods, but there is not enough hours in the day to progress as fast as I would like. Thats about it for now, kinda busy elsewhere so SWIM is gonna fruit these out and get back to ya with some experiments for the next batch.
cublium:
Do spores spores stay unharmed by sub zero C temperatures?Cub ordered some from US and wants to try some shroom growing.
Urupeh:
Swim like to make adventures to colecting psilo-shrooms in all over his coutry, and he notice (apart from the individual strain diferences)that in the regions of lower temperature and in the colder times of the year, the mushrooms apear to be stronger, long time ago in his dreams swim cultivate on every grain he could find and in compost made whith horse shit, and in the colder months the same strain apear to be more powerful in the desired effects. (just to clarify swim in his weird dreams was living in a country of tropical and subtropical clima). By experience swim thing it is more easy and funny to collect than to grow when the interrest focus on extraction, but growing is better if the intend of the experience is to eat the raw (or dreid)mushrooms, because sanitary and higienics play an important role.
Mr_Bronson:
cublium: received wisdom on the preservation of hydrated spores (spore syringes) seems to be: do not freeze them (refrigerate only). I haven't tried freezing, but bacterial spores survive - why not fungal?
aroit_synth: the liquid medium became contaminated: smelt ike yeast and was bubbling like crazy. Was using honey amongst other things, so maybe I could have made some hallucinogenic mead. Used it to fertilise the compost heap. Will try again this weekend with more H2O2. I'm careful with sterility: bad luck or bad procedure somewhere.
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