okey dokey...
SWIM acquired itself some acacia obtusifolia bark, and extracted a quantitiy of it, using good quality chemistry equipment, a good quality pH meter form a hydro shop calibrated within a week of use, a 1L sep funnel, a buchner filter with good suction (though SWIM suspects this was not that important, though helpful).
etc
okay, the big deal was this. the extraction was done with a double boiler, the extract was not permitted to rise above water bp. 1.5 hours. first run.
this acidic extract was filtered with the aforementioned buchner vacuum filter, first with a coarse grade filter, and then with a more fine grade filter.
then, SWIM defatted with 1x wash of toluene, 3x wash of xylene (approximatley 100ml each). about 100ml of naphtha to clear the whatever was left behind by the last four washes, and ...
basified to 12.8. Extracted in the first instance with naptha, and then washed with standard tapwater (measured at pH ~9) and an interesting phenomenon occurred
the yellow colour which the naptha acquired after the extraction was completely washed out into the water (at aforementioned pH 9).
further to this SWIM promises me, more stories...
however it is rather interesting... 4x defatting with aromatic solvents, and then a final wash with naptha, and then, to the polar extract, a wash with water, which was obviously around pH 9, more likely to be 8.8 than 9, however 9 was what SWIM sweared the pH meter said. the naptha turned yellow after the extraction, and then the yellow was gone in the polar wash....
what could this mean???
dang, I am gonna have to hassle the SWIM about further stories.
oh...
SWIM reported more...
it then happened again with a new naptha extraction and a further wash of the tap water (which as mentioned, took up all the yellow colouration)...
And the second wash got rid of the colour completely...
omg
well, anyway, SWIM has further to tell on his imaginary adventures.
but anyway, he found this colour migration to be quite intriguing...
SWIM promises more, but he's the most unreliable SWIM I have ever known
dang