Author Topic: common LSD reagents  (Read 758 times)

Vesp

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Re: common LSD reagents
« Reply #20 on: April 14, 2009, 10:10:56 PM »
I'd guess tryptamines, so I would think you could add tryptophan, or something similiar to help it along but who knows, I have heard adding tryptamine compounds to magic mushroom grows can increase the mushroom's potency, but this could just be an urban legend.
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poisoninthestain

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Re: common LSD reagents
« Reply #21 on: April 14, 2009, 10:28:28 PM »
Any derivative of indole will do.

In my microbiology class we used to use tryptophan and indole as the main ones with a nitrogen source like ammonium nitrate in agar slants.


Vesp

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Re: common LSD reagents
« Reply #22 on: April 14, 2009, 10:35:59 PM »
That is good to hear that any indole derivative will work, but do you know if it will actually increase the wanted alkaloid content? I guess adding indoles would at least ensure that it will have adequate resources, if not increase the alkaloid content through some other process. 
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Sedit

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Re: common LSD reagents
« Reply #23 on: April 14, 2009, 11:11:05 PM »
[1]
Quote
Psilocybin and psilocin belong to the tryptamine (indoleamine) family of psychedelics. They occur naturally in many mushrooms. The species of Psilocybe mushrooms have high a capacity to transform fed tryptamine into a psychoactive ingredient psilocybin by a methylation and hydroxylation reaction. Studies of psilocybin biosynthesis in mushrooms show that the amino acid tryptophan and tryptamine are precursors to the indole alkaloids (Gartz 1989). Although psilocybin can also be chemically synthesized, it is very difficult and expensive. (Cuomo 1994).

Im sure the capacity vs toxicity to the fungi could appear if you tryed to over do it with the tryptamines but surely you could increase the potancy dramaticly of ergot alkaloids seeing as they are both from the same basic structure. With ergots though there would have to be some other substance added to build off the structure I would assume.

Judging from[2] It appears methyl methonine plays a role in the biosynthesis also.

[1]:http://www.shroomery.org/6231/Psilocybin-awareness
[2]:http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1177782
« Last Edit: April 14, 2009, 11:29:39 PM by sedit »
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Mr.Murphy

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Re: common LSD reagents
« Reply #24 on: August 06, 2009, 04:57:20 PM »
Vesp- There's dozens of different strains of ergot but only a few that have appreciable amounts of lysergamides in them.

The refs. are out there if you're interested.
I'm indeed interested!

Mr.Murphy

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Re: common LSD reagents
« Reply #25 on: August 06, 2009, 05:05:29 PM »
I'd guess tryptamines, so I would think you could add tryptophan, or something similiar to help it along but who knows, I have heard adding tryptamine compounds to magic mushroom grows can increase the mushroom's potency, but this could just be an urban legend.
Not a legend but reported by Jochen Gartz in Journal of Basic Microbiology 1989, 29(6), 347-352.

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Vanadium

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Re: common LSD reagents
« Reply #26 on: August 06, 2009, 06:48:30 PM »
I'd guess tryptamines, so I would think you could add tryptophan, or something similiar to help it along but who knows, I have heard adding tryptamine compounds to magic mushroom grows can increase the mushroom's potency, but this could just be an urban legend.
Not a legend but reported by Jochen Gartz in Journal of Basic Microbiology 1989, 29(6), 347-352.

- Murphy

He has a patent on this too. The Shroomery has plenty of information on this with regards to mushrooms. To sum up the immediately obvious information, adding tryptophan to the growth culture increases 4-OH-DMT content slightly. Adding tryptamine increases it significantly, and every other compound in range of possible biosynthetic pathways increases it by about the same amount as tryptamine (i.e. NMT, DMT, 4-OH-tryptamine, etc). Researching this is a great way to waste a day--added bonus, mushrooms have been shown to hydroxylate foreign alkylated tryptamines as anyone who has read TiHKAL knows.

BTW, a quick google search turned this up: http://mic.sgmjournals.org/cgi/reprint/118/2/485.pdf
But honestly, I doubt very much LSD is made from fungi.

Sedit

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Re: common LSD reagents
« Reply #27 on: August 06, 2009, 08:04:08 PM »
Quote
BTW, a quick google search turned this up: http://mic.sgmjournals.org/cgi/reprint/118/2/485.pdf
But honestly, I doubt very much LSD is made from fungi.

Im confused as to what you mean? That text does not speak of LSD but of ergot alkaloids which are the precursors to LSD.
Why exactly would you doubt that LSD is made from Fungi when the most common of all starting material arises from the Ergot fungus?
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Vanadium

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Re: common LSD reagents
« Reply #28 on: August 06, 2009, 08:34:07 PM »
Quote
Im confused as to what you mean? That text does not speak of LSD but of ergot alkaloids which are the precursors to LSD.
I know, that was in relation to the above discussion on increasing alkaloid content by adding certain amino acids or precursors.
Quote
Why exactly would you doubt that LSD is made from Fungi when the most common of all starting material arises from the Ergot fungus?
Just because something is common doesn't mean it'll be used. Vanillin is far more common than safrole but MDMA is rarely made through the former. Using fungus just isn't practical in when compared to diverting ergot alkaloids from medicinal use. Not all clandestine chemists think only in terms of OTCness like you, Sedit. ;)
« Last Edit: August 06, 2009, 08:36:12 PM by Vanadium »

Vesp

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Re: common LSD reagents
« Reply #29 on: August 06, 2009, 08:37:41 PM »
Really it would also depend on how much of it is being made at a time.
Someone might happen to have large quantities of HWBR and that might be the most practical for them, while someone who is producing large quantities of LSD might have to use an ergot culture in a 55 gallon drum.  :P
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quetzalcoatl

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Re: common LSD reagents
« Reply #30 on: December 07, 2009, 01:29:54 AM »
Personally, I don't see the problem with starting from morning glories, and seperating the alkaloids with chromatography.

Why go to all this trouble to locate ergot, care and culture it, then extract the alkaloids? any old Lysergia will do, as long
as you can get it to an anhydrouse lysergic acid salt. (which is very possible.)

I don't know if you've ever cultured mushrooms, vesp, but its a pain in the fucking ass when cultures go bad.
Especially with something as seemingly tempremental as ergot, which really wants a living grain, has tiny fruitbodies,
and is fuck impossible to obtain.

Identifying a propper ergot strain from the wild is really not my forté. Personally I find it difficult to accurately identify species because you're never quite sure what you've got.
There could be any number of variations from what you've read or seen. Just look at p. semilanceata;
wildly variable. You get those little tiny brown seethrough ones, when they're young, right through to 5 - 6 inch
monsters, which are tan with brown gills, and usually by that size they're looking a bit shaggy around the edges!

One thing entirely sitting on the net looking for information and discussing, but another entirely going out and identifying
the species in question, cuturing it with acceptible losses due to contamination, and then extracting alkaloids from it.

IMHO, just buy the seeds. Its far more reliable.

xxxxx

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Re: common LSD reagents
« Reply #31 on: September 23, 2010, 09:09:58 PM »
FYI ergocristine is available at a very reasonable prices from Asian suppliers. I got a couple of quotes for under one dollar a gram for a 1kg sample. Very reasonable if you ask me ;) when you have a possibility of synthing maybe 300grams of LSD from it. It just feels very satisfying holding in your hands something you know people would pay A LOT of money for :)

jon

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Re: common LSD reagents
« Reply #32 on: September 24, 2010, 01:16:52 AM »
you know i wonder why windowlycker got it up the ass for buying 10 grams of ergocrystine when it had'nt been scheduled at the time of his arrest?
is there some sort of retroactive/unwritten law involving the ergopeptides?

Vesp

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Re: common LSD reagents
« Reply #33 on: September 24, 2010, 01:20:02 AM »
Perhaps the full story has not been told.
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jon

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Re: common LSD reagents
« Reply #34 on: September 24, 2010, 01:36:51 AM »
probably a mole informant type situation

embezzler

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Re: common LSD reagents
« Reply #35 on: September 24, 2010, 09:44:44 AM »
I would imagine that they could show intent rather than just the possession.
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jon

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Re: common LSD reagents
« Reply #36 on: September 24, 2010, 03:53:25 PM »
from what i understand he bought it from some company in the eu. that likely informed customs who informed the dea

director of sound

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Re: common LSD reagents
« Reply #37 on: October 10, 2010, 07:38:28 AM »
here is a short list of lysergamide containing taxa ive come up with, there are more but ill have to dig in my books again...


Acremonium coenophialum: ergine

Aspergillus fumigatus: elymoclavine

Argyreia nervosa: ergine (22.68%), isoergine (31.36%), lysergic acid hydroxyethylamide (10%)   .3% TA 

Balansia epichloe: ergonovine, elymoclavine

B. claviceps: ergonovine

B. henningsiana: ergonovine

Claviceps africana: elymoclavine  (ALL CLAVICEPS .5 - 2% TA)

C. fusiformis: elymoclavine, lysergene, lysergol

C. imperatae: ergonovine

C. litoralis : ergonovine, lysergine, lysergol, elymoclavine

C. microspora: elymoclavine

C. miscanthi: ergonovine, elymoclavine

C. paspali: ergonovine, ergine, isoergine, elymoclavine, Lysergic acid hydroxyethylamide

C. purpurea: ergonovine, ergine, isoergine, elymoclavine

C. purpurea var sasae: ergonovine, elymoclavine

C. cynodontis: ergonovine

C. gigantea: elymoclavine

Ipomoea violacea: ergine .01 - .02% TA

Stipa robusta: ergine 35ug/g

Turbina corymbosa: ergine .04% TA
 

as for PyBOP ive seen it on ebay a few times fairly cheap around $150 for 100g.   bromocriptine here: http://www.antiaging-systems.com/PRG-172/bromocriptine-parlodel.htm at $24 for a 30ct bottle of 2.5mg tabs

jon

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Re: common LSD reagents
« Reply #38 on: October 10, 2010, 07:45:08 AM »
director of sound i have a few methods that are chemoselective i'd like to share with you for dehalogenation of aryl systems.
this one i like in particular.
my question to you is how would the 9-10 conjugated double bond respond to the lewis acid zinc chloride?
can you predict if there would be a rearrangement to a napthelene type system?
this system is mild conducted at room temperature in a biphasic system of thf/ammonium chloride and zinc
the mechanism is simple the ammonium chloride activates the zinc the thf ligands the metal cation which metalates the aryl halide the arylzinc halide is then reduced by ammonium chloride.
what do you think?
also i hear the peptide coupling methods are'nt all they're cracked up to be because they are water sensitive is it true anhydrous lysergic acid has to be used in the peptide coupling routes?

http://rapidshare.com/files/424170765/Zn_NH4Cl.pdf

^palladium no way in hell are you going to get ergoanything for $1 per gram try at least 15 more realistically 26-50 dollars per gram.
« Last Edit: October 10, 2010, 08:13:03 AM by jon »

director of sound

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Re: common LSD reagents
« Reply #39 on: October 10, 2010, 08:17:52 AM »
the peptides are not too bad and anhydrous lysergic acid can be had by heating lysergic acid monohydrate under  a dry N2 atmosphere at 1.5-2in mercury to about 80*F for about 30m per a gram. the advantage they have is that nearly no ISO-LSD is produced and often chromo is not even necessary to get a pure product twards the 96-98% range. i could see your method having and advantage over grignards because of the easily obtainable chems, id just be worried about a chlorinated intermediate  forming and if there are any stray Cl- ions your yields could be hurt drastically.  the lewis acid could cleave the tripeptide from the molecule at the N-C(OH) juncture with the amide but you'll still have the bromine to deal with. stray zinc in the rxn could take up the bromine but something would have to donate a carbon otherwise the whole molecule  is going to fall apart. if you started with lysergic acid instead of bromocriptine i could see it working.