Author Topic: Crude Psilocybin Extraction Tek  (Read 254 times)

agmotes165

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Crude Psilocybin Extraction Tek
« on: February 19, 2009, 01:46:09 AM »
This a way use the spent cakes or bulk substrates that will no longer fruit. A great way to squeeze out the last little bit of actives out of your projects.

WHAT YOU WILL NEED:

1. Dry Mycelium material or mushroom material, doesnt matter
2. Something to grind it up with (magic bullet, coffee grinder, etc.)
3. Methanol (paint section of hardware store)
4. Glass containers big enough for whatever size job you are gonna be doing
5. Filters (coffee filters or filter paper)
6. Funnel
7. Large bucket or bowl for the hot water bath

Method

1. Grind up dried mushroom or mycelium material to fine powder

2. Combine mushroom powder to methanol in proportions of 10mL methanol for every 100mg mushroom powder.

3. Place container with mushroom methanol slurry in hot water bath for 4 hours

4. Filter slurry, save the solids for next extraction, and save the liquid in separate container.

5. Add fresh methanol to solid portion and place in hot water bath for 4 hours.

6. Repeat filtration and extraction once more, for a total of 3 volumes of liquid methonal. You may now discard the solid material.

7. In an open container evaporate liquid to total dryness using the hot water bath. End product after evaporation is psilocybin extract.

This extracted material should contain 25-50 percent psilocybin and psilocin. Every 100 grams of dried mycelium/mushroom tissue should yield about 2 grams of this psilocybin/psilocin residue, which will contain a total of 500mg of psilocybin/psilocin or approximately fifty 10mg doses.  This means that a good dose of this residue is about 40 mg of residue.

Stay tuned...more to come  8)
Peace to all them kids...
agmotes165

Douchermann

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Re: Crude Psilocybin Extraction Tek
« Reply #1 on: March 11, 2009, 07:39:03 AM »
Seems a bit heavy on the solvent side.  10g of dried mushrooms = 1L of methanol?  Consider psilocybe cubensis contains about 5.5mg of psilocybin and 0.5mg of psilocin per gram of dried mushroom (IIRC) then that seems quite wasteful, unless of course it's distilled and kept. 

As usual, I always recommend the use of food grade ethanol for any product that will be ingested, as opposed to the harmful methanol.  It's most definitely not necessary, as you can handle a little bit of methanol and it's not a 'cumulative' poison, but it's always better to be safe than sorry.  Good write-up none the less.

shroomedalice

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Re: Crude Psilocybin Extraction Tek
« Reply #2 on: November 22, 2009, 05:50:22 PM »
I was looking into this and found this post to shulgin that might be of interest
http://www.cognitiveliberty.org/shulgin/adsarchive/extraction.htm

Sedit

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Re: Crude Psilocybin Extraction Tek
« Reply #3 on: November 22, 2009, 06:00:39 PM »
That is pretty cool and I always assumed that the Psilocybin would be soluble in EtOH not giving it much thought. If we take what shulgin says here then purification should be as easy as extraction with an EtOH/H2O mixture then azeotropic distillation to remove the H2O use. Doing so should cause precipitation of the Alkaloid simular to a duel solvent recrystalization while still allowing the other unwanted contamination to stay solvated in the EtOH.

I would have to assume here that the addition of Ether to this could also possibly precipitate the alkaloids as well albeit with a small amount of loss.
There once were some bees and you took all there stuff!
You pissed off the wasp now enough is enough!!!

shroomedalice

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Re: Crude Psilocybin Extraction Tek
« Reply #4 on: November 22, 2009, 06:15:46 PM »
then all you have to do is put it in a sugar cube and you have shroomedalice hehe :)

I love my shrooms.