Claviceps purpurea yield confusion cleared (high bandwidth)

Nitroxamide · Mon Jul 11, 2005 6:09 pm

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timnickbear · Mon Jul 11, 2005 11:57 pm

guess I am going to have to get the old biotech of filamentous fungi book out for all of you to see how to correctly do the culture as of recent, not saying this liturature isn't informative. The process is better done in front of a laminar pref to avoid contams, and usually when stock culture isn't used gentamycin sulfate is used in agar to kill off bacteria allowing for a sterile culture to be obtained. A t-s slant is pref to get it to grow before liquid culture is innocculated by the fungus.

Nitroxamide · Tue Jul 12, 2005 1:32 am

That would be awesome, please do. Very Happy

Before all this, it seemed like some people randomly said claviceps worked great and some randomly said it didn't.

timnickbear · Tue Jul 12, 2005 4:31 am

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15904941&query_hl=2
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=DisplayFiltered&DB=pubmed
http://www.blackwell-synergy.com/doi/full/10.1111/j.1364-3703.2004.00237.x
that last one should keep you interested until I get this sucker into pdf.

Alice · Tue Jul 12, 2005 8:08 pm

well i can put my 2 cents also into this one
here it is

Optimization_of_Conditions_for_Producer_of_the_Ergot_Alkaloids

enjoy
@lice

timnickbear · Wed Jul 13, 2005 2:17 am

Can some one please find:
Mantle, P. G. (1975) in The Filamentous Fungi: Vol. 1, Industrial Mycology (Smith, J. E., and Berry D. R., eds.), pp. 281-300, John Wiley, New York.

timnickbear · Wed Jul 13, 2005 2:22 am

Finkelstein, D.B. & Ball, C., eds. (1992). Biotechnology of Filamentous Fungi: Technology and Products. Butterworth-Heinemann, Boston. pp. 265-289

timnickbear · Wed Jul 13, 2005 2:22 am

Those should get you off into a good read Smile

Alice · Wed Jul 13, 2005 4:43 am

well dono if thats exactly what u looking for but its good one and more specific one than Biotechnology of Filamentous Fungi

here u go

Biotechnology_and_genetics_of_ergot_alkaloids

timnickbear · Thu Jul 14, 2005 2:23 am

oh wow thats a good read. The one by mantle describes how to get 5mg/ml of erogtamine in a submerged culture. More of the actual nutrient workup. You had a good read though. Can we get that in the archives?

brain · Linguist Extraordinaire

Can enyone have more pages form book from 1-st post ??? mabey 190...x pages ? there is writen how to select claviceps culture... mabey someone know how to do it? to indicate and select culture that giwes highest yelds of ergotamine?