The above picture shows the TLC analysis of the reaction product
between two reagents, “3BMB” and “TBT”.
The plates were spotted across the bottom with the two
starting materials (3BMB & TBT) along with the reaction
residue, and eluted with either 100% hexanes or 90/10
hexanes and ethyl actetate.
The two plates on the left where photographed with a
hand held digital camera while illuminating the two plates
with UV light. The two plates on the right are an image
enhanced version of the UV photo on the left. This image
enhancement was done using the freeware program
“Scion Image” available here.
Enhancement was done by converting to gray scale and
adjusting for contrast and brightness.
Based on the above, what conclusions can be made about
the reaction results?
How would you isolate the products “A” and “B” if the reaction
residue weighs about 1 gram?
I would try column chromatography, use hexane 100% solvent to wet your silicate column then mix, using the same solvent ratio( hexane/ethyl acetate), your product and pour into your column , you may need a black light to see the second and third bands and collect and separate from start of band to the start of the next band of the wanted unknowns.......java
I was wondering if there is a way to see the color of the bands as they travel down the silicate column , hence one can eye the compound already established at what level it is by the TLC that is done prior to the column chromatography. In small samples I can see the only way to track the wanted compound is to tlc periodically to make sure one catches all of the compound , but the time that it takes to develop the tlc may be too long , hence that why I would prefer an additive to the eluent to florece with a UV light...........by the way the pdf to flash chromatography is very good ...........java
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