LSD chromotography photos

wyndowlicker · Member of the Month

HEy now,

Here is a photo for you guys to check out.A bee sent this to me and wants me to compile a more reasonable write up from different steps of a specific process.I am all to happy to do this.There is quite a bit of data that is outdated or completely untrue.Also, some fokelore that can be layed to rest.More to come later.

Vitriodor · Sat Feb 26, 2005 5:50 am

If you want to store your LSD in urine you better use amber glass containers and don´t illuminate it with strong UV light Wink

AN ANALYSIS OF LSD IN URINE UNDER VARIOUS STORAGE CONDITIONS
McNally A.J., Li Z., Pilcher I., Salamone S.J.

Roche Diagnostic Systems, Inc., 1080 US Highway 202, Somerville, NJ 08876, USA

--------------------------------------------------------------------------------
Few formal studies have been conducted on the stability of LSD in urine under various storage conditions. A controlled study was undertaken to determine the stability of LSD in pooled urine samples at 0.5 ng/mL and 500 ng/mL of LSD. The concentrations of LSD in urine samples were followed over time at various temperatures, in different types of storage containers, at various exposures to different wavelengths of light, and at varying pHs. LSD concentrations were measured quantitatively by the Abuscreen RIA and by HPLC using a fluorescence detection method. Good correlation of data was observed between the immunoassay binding and the fluorescent integrity of the LSD molecule. Thermostability studies were conducted in the dark in amber glass and polyethylene containers and demonstrated no significant loss at 25° C for four weeks. At higher temperatures there was a 30% loss at 37° C and up to a 40% loss at 45° C for four weeks. These thermostability results were the same for each of the container materials tested.
Three container types, amber glass, polyethylene, and quartz, were used as storage containers for testing the stability of LSD during light exposure. Various light conditions used were: 15 watts of fluorescence light at 15 cm or 200 cm distance, 254 nm UV light with 345 watts, and 365 nm light with 345 watts. All light exposure studies were conducted at various time points at 25° C. The urine containing LSD in the amber glass showed no loss under any light conditions. The polyethylene container after one month revealed a 20% loss when placed 200 cm from the fluorescence source (normal lab conditions) and displayed a 60% loss when placed 15 cm from the fluorescence source. In addition, when using the polyethylene container at a distance of 15 cm from a 254 nm strong UV light source, no loss was observed after eight hours. When this polyethylene container was placed 15 cm from a 365 nm strong light source for eight hours, a 50% loss was observed. In the quartz container, losses of 28% and 50% were observed usig strong light sources of 254 nm and 365 nm respectively, at a 15 cm distance for eight hours.

This study demonstrates the importance of proper storage conditions of LSD in urine in order to insure proper analytical testing results over time.

wyndowlicker · Member of the Month

Hey now,

Now that ive figured out how to use my ftp crap properly I dug this one up.Its a much further back from the column.Nice seps due to good column packing techniques. Evil or Very Mad

IndoleAmine · Dreamreader Deluxe

This one hasn't moved much compared to the first pic! And was made at the same time... (no pics of further diluted columns? Wanna see more!) Shocked

Which solvents were used for dilution? Which adsorbent, alumina or silica? Times? (a complete writeup, after all?) Cool

i_a

wyndowlicker · Member of the Month

HEy now,

That is a close up and a shot from further back.I wasnt there but thats what im told. Wink

I have an idea it was activated alumina activation II-III.DCM was used.I will get a very detailed work up from LSA.

Laughing

DAY

NIGHT

IndoleAmine · Dreamreader Deluxe

the writeup - can't await it...
(awesome!)

tone3721 · Sat Mar 26, 2005 8:10 am

I think I just shit my pants. A write up too???????

n00dle · Sun Apr 10, 2005 2:07 pm

bye bye synthetikal.

QuickFitAbuser · Quality Control

Well, this certainly has something to say for those persons who drink urine.
I recall reading about persons who would drink the piss of persons who had consummed a type of mushroom 'fly agaria' or fly amarita', cant bee sure, and still experienced a similiar effect, abiet a bit salty.

Hmm storing amphetamine under urine: Sure oficer, this is just my piss frozen, for health reason's, sure you can take a sample, as long as I watch. Miss .

nubee · Master Archiver

ive drunk my urine before, a whole glass when it was real clear, while under the inffluence...

it was nice, in a way that you think oh that's nice i was expecting something rank and bitter... Wink

-try it, it did good things for me...

IndoleAmine · Dreamreader Deluxe

gulp!!

wyndowlicker · Member of the Month

HEy now,

Thats foul dude.Unless your drinking it in an amanita muscaria ritual.CDI is the coupler.

W.L Razz

IndoleAmine · Dreamreader Deluxe

Might I also ask about the solvent used? (DMF maybe?) Wink

(and why has this become the official "drink your piss" thread?
AFAICS its not really related to the original topic at all..... Laughing

)

i_a

wyndowlicker · Member of the Month

Hey now,

Yes DMF even though it is a real bitch in a rotovap to get it back.From what i was told you have to have a dri ice dewar inline with a refridgerated trap in order to not destroy your pump.Unless you have one bad ass teflon pump.

W.L Razz

IndoleAmine · Dreamreader Deluxe

Whoah! maybe running acetone/dry ice through the rotovap condenser would help too - or you could rig up something with a modified cooling pump running refrigerant through your condenser instead your refrigerator.. Laughing

..dry ice dewar to cool the receiver, AND another cooling trap in line? sounds like a messy and fast process *yuck*..
I thought DMF would boil around 150°C at atmos? Does it evaporate that damn fast that you need such fancy cooling stuff?

how good is the vacuum that is used by the guy who has done this; do you know it by coincidence? (just curious)