Tips and Suggestion, Question and Answer (included )

java · Consumer

This will be a thread were everyone is invited to share a tip or a suggestion and a question with an answer included... that has been handy in the lab ........java

so lets get started......

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Question:

Are there any "tricks" for developing and visualizing spots when DMSO is used as a reaction solvent? DMSO just seems to spread out on the plate rather than evaporate, even after prolonged heating. As I result I often have huge spots that obscure a component or two, no matter what solvent system or adsorbent (normal or reverse phase) I use. The reaction components are usually not UV-active.

Answer:

This is a very common problem, especially with solvents such as DMSO, DMF and HMPA. The trick I use it as follows:

Take a small aliquot of your reaction, say 50 microliters and dissolve it
in a solvent such as ethyl acetate or dichloromethane. then add 250-500
microliters of water. Shake the mixture than use the organic layer and spot your TLC. This mini extraction usually removes most of the DMSO from your sample, so the TLC is usually cleaner.

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