Ephedrine producing method by using large-scale.....

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Ephedrine producing method by using large-scale ephedra cell culture as material
Inventor(s):CHA LIHANG (CN); JIAO YUXIA (CN); LIU DALU (CN)
Patent Number:CN1256316
Publication date:2000-06-14
Applicant(s)::INST OF CHEMICAL METALLURG CHI (CN)
Requested Patent:CN1256316 Application Number:CN19980125186 19981204 Priority Number(s):CN19980125186 19981204 IPC Classification:C12P13/00 EC Classification:EC Classification:Equivalents:CN1077600B

Abstract The present invention relates to the method of producing ephedrine by using large-scale ephedra cell ZHJ-25 CGMCC No.0359 culture as raw material. Ephedra cell ZHJ-25 CGMCC No.0359 is inoculated intosugar containing culture medium through three ways, and cultured in 25 +/- 2 deg.C for 20-50 days before the culture is dried at 60 deg.C to obtain ephedrine-containing raw material. The method is simple, may be used in continuous or semi-continuous industrial production of ephedrine in high yield and the product has stable ephedrine content.

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Here is a related article as many will be posted on this thread as to acquire the ephedrine culture ....also a translated copy of patent is being searched for.....java

Plant Cell Cultures - An Alternative and Efficient Source for
the Production of Biologically Important
Secondary Metabolites
Vanisree Mulabagal and Hsin-Sheng Tsay
International Journal of Applied Science and Engineering
2004. 2, 1: 29-48
pdf

Abstract: Plant cell culture systems represent a potential renewable source of valuable medicinal
compounds, flavors, fragrances, and colorants, which cannot be produced by microbial cells
or chemical synthesis. Biotechnological applications of plant cell cultures presents the most updated
reviews on current techniques in plant culture in the field. The evolving commercial importance
of the secondary metabolites has in recent years resulted in a great interest, in secondary
metabolism, and particularly in the possibility to alter the production of bioactive plant metabolites
by means of cell culture technology. The principle advantage of this technology is that it
may provide continuous, reliable source of plant pharmaceuticals and could be used for the
large-scale culture of plant cells from which these metabolites can be extracted. In addition to its
importance in the discovery of new medicines, plant cell culture technology plays an even more
significant role in solving world hunger by developing agricultural crops that provide both higher
yield and more resistance to pathogens and adverse environmental and climatic conditions. This
paper describes the callus and suspension culture methods that we have established in our laboratory
for the production of bioactive secondary metabolites from medicinal plants.

Keywords: bioreactors; callus cultures; secondary metabolites; suspension cultures; pharmaceuticals;
precursor feeding.

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Studies on the production of some important secondary
metabolites from medicinal plants by plant tissue cultures
Mulabagal Vanisree1, Chen-Yue Lee2, Shu-Fung Lo2,3, Satish Manohar Nalawade1, Chien Yih Lin3, and
Hsin-Sheng Tsay*,
Bot. Bull. Acad. Sin. (2004) 45: 1-22
pdf

Abstract.
Plants are a tremendous source for the discovery of new products of medicinal value for drug development.
Today several distinct chemicals derived from plants are important drugs currently used in one or more countries in
the world. Many of the drugs sold today are simple synthetic modifications or copies of the naturally obtained
substances. The evolving commercial importance of secondary metabolites has in recent years resulted in a great
interest in secondary metabolism, particularly in the possibility of altering the production of bioactive plant metabolites
by means of tissue culture technology. Plant cell culture technologies were introduced at the end of the 1960’s
as a possible tool for both studying and producing plant secondary metabolites. Different strategies, using an in vitro
system, have been extensively studied to improve the production of plant chemicals. The focus of the present review
is the application of tissue culture technology for the production of some important plant pharmaceuticals. Also, we
describe the results of in vitro cultures and production of some important secondary metabolites obtained in our
laboratory.
Keywords: Biotransformations; Cell suspension cultures; Hairy root cultures; Pharmaceuticals; Secondary metabolites.