Can we find a way to get E. coli to produce lysergic acid?
Re: Have you heard of the THC E.Coli?
« Reply #20 on: May 02, 2011, 03:16:33 AM »
Topic: Have you heard of the THC E.Coli? (Read 300 times)
psychexplorer
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Vesp
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Re: Have you heard of the THC E.Coli?
« Reply #21 on: May 02, 2011, 03:22:27 AM »
If you could isolate the gene cluster from the claviceps, and than got it to incorperate into a plasmid that than gets taken into the DNA sequence of the E. Coli - that'd do the trick - but its pretty much out of our reach AFAIK, but perhaps not?
Also look into that calcium phosphate HEPES solution to take in DNA fragments...
Also look into that calcium phosphate HEPES solution to take in DNA fragments...
OoBYCoO
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Re: Have you heard of the THC E.Coli?
« Reply #22 on: May 02, 2011, 03:28:25 AM »
I love that ergot avatar Vesp!!! 
psychexplorer
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Re: Have you heard of the THC E.Coli?
« Reply #23 on: May 02, 2011, 03:49:11 AM »
Biochem isn't my thing, so I wouldn't have the first idea where to start, or even if it is remotely possible.
IIRC natural ergoline production begins with tryptophan and a few other compounds. Wouldn't the engineered E. coli have to be modified to produce the precursors, in addition to being modified to carry out final assembly? Might there be a possibility that something important is substrate specific?
The need for an accessible precursor to the world has been haunting my dreams lately...but I'm not up to the task. That hurts to echo over and over.
IIRC natural ergoline production begins with tryptophan and a few other compounds. Wouldn't the engineered E. coli have to be modified to produce the precursors, in addition to being modified to carry out final assembly? Might there be a possibility that something important is substrate specific?
The need for an accessible precursor to the world has been haunting my dreams lately...but I'm not up to the task. That hurts to echo over and over.
akcom
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Re: Have you heard of the THC E.Coli?
« Reply #24 on: May 02, 2011, 03:57:20 AM »
It's not just a simply an issue of making the bacterium produce your compound. It has to produce it in sufficient quantities to make the whole process economically feasible. It also desirable to export the compound from the cell. From what I understand, the tagging/untagging process is complex and not completely understood and periplasmic export remains a challenge
Sedit
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Re: Have you heard of the THC E.Coli?
« Reply #25 on: May 02, 2011, 04:06:30 AM »
Even if one managed to extract, plasmidize(<- is that even a word), and incoorperate the DNA into a bacteria there is the issue as to if it will be expressed.
Vesp
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Re: Have you heard of the THC E.Coli?
« Reply #26 on: May 02, 2011, 04:38:25 AM »
I think the claviceps genus will become a viable route to lysergic acid in time, or one of its relatives. So we ought not worry about E. coli. 
akcom
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Re: Have you heard of the THC E.Coli?
« Reply #27 on: May 02, 2011, 06:13:21 AM »
Even if one managed to extract, plasmidize(<- is that even a word), and incoorperate the DNA into a bacteria there is the issue as to if it will be expressed.Thats why they attach the insulin operon to the B-galactosidase gene
The Lone Stranger
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Re: Have you heard of the THC E.Coli?
« Reply #28 on: May 02, 2011, 08:01:14 AM »
LOL guys what fun fantasys......
Some loose disordered thoughts ----->
At the end of the report from the university of dortmund there is an interesting line "Im Rahmen unserer Arbeiten wollen wir in der Zukunft ein Stück weiter gehen und die humanen Enzyme als Gene in die Mikroorganismen bringen, die bereits THC herstellen«" = Within the limits of our work we want in the future to go s step further and insert the human enzymes that already produce THC as genes into the microorganism .
Thats crap , especialy as it comes from a "scientist" , because the human body doesnt produce THC . It produces A THC . = a BIG difference . The first being a specific chemical = T.H.cannabinol and the other being a specific T.H.cannabinoid . But anyway what a wonderfull fantasy....... one could swallow a few drops containing enzymes and the goodys get produced in ones stomach / arse !!!! Oh god Oh god one has fantasys of the pigs aplying for a warrant to search ones stomach !!!!! And then the film where they shrink people and send them through a persons blood stream . Then a picture of 16 7 foot pigs being shrunk and invadeing ones system with cocked guns at 3 in the morning. Then the thought " Well if they shrunck the fuckers to that size their bodys would then match their brain size now........."
Then the thought ...... the body produces DMT and opioids......
I think that some things need clearing up . They say the plant contains 0.2 % THC and that smokeing grass can contain up to 25 % . This is total bollocks . Why ? Because they dont say what the % is of = the sum total in the plant , the oil or part of the THC group in the oil ? Its physicaly imposible that they are talking about the plant because if it was true the plants would melt and flow away if the weather got warm ........ I think that the 25 % is the ammount of THCannabinol in the TCcannabinoids in the oil ?
Next . The report says as a justification for the new method that at the moment they can produce 20 kgs of THC a year but the need is for 1 ton a year !!! Sounds strange to me . What the fuck could they do with either quantity as experimentation with THC is somewhere between totaly prohibited and next to impossible to get permission to experiment with . Plus there were about 3 years ago only 10 - 15 people in germany that were allowed to use THC as a medicine . Can someone please work that out ? I mean 1 ton divided by the number of people in germany , the amount one needs to get zapped and then days . Sounds to me like germany would be permanently floating comatose about 3 foot over the ground ......
As far as how they get the genes into the microorganisms goes they use three diferent words = plant , insert and bring . Other than that i cant see any more info about it .
Some loose disordered thoughts ----->
At the end of the report from the university of dortmund there is an interesting line "Im Rahmen unserer Arbeiten wollen wir in der Zukunft ein Stück weiter gehen und die humanen Enzyme als Gene in die Mikroorganismen bringen, die bereits THC herstellen«" = Within the limits of our work we want in the future to go s step further and insert the human enzymes that already produce THC as genes into the microorganism .
Thats crap , especialy as it comes from a "scientist" , because the human body doesnt produce THC . It produces A THC . = a BIG difference . The first being a specific chemical = T.H.cannabinol and the other being a specific T.H.cannabinoid . But anyway what a wonderfull fantasy....... one could swallow a few drops containing enzymes and the goodys get produced in ones stomach / arse !!!! Oh god Oh god one has fantasys of the pigs aplying for a warrant to search ones stomach !!!!! And then the film where they shrink people and send them through a persons blood stream . Then a picture of 16 7 foot pigs being shrunk and invadeing ones system with cocked guns at 3 in the morning. Then the thought " Well if they shrunck the fuckers to that size their bodys would then match their brain size now........."
Then the thought ...... the body produces DMT and opioids......
I think that some things need clearing up . They say the plant contains 0.2 % THC and that smokeing grass can contain up to 25 % . This is total bollocks . Why ? Because they dont say what the % is of = the sum total in the plant , the oil or part of the THC group in the oil ? Its physicaly imposible that they are talking about the plant because if it was true the plants would melt and flow away if the weather got warm ........ I think that the 25 % is the ammount of THCannabinol in the TCcannabinoids in the oil ?
Next . The report says as a justification for the new method that at the moment they can produce 20 kgs of THC a year but the need is for 1 ton a year !!! Sounds strange to me . What the fuck could they do with either quantity as experimentation with THC is somewhere between totaly prohibited and next to impossible to get permission to experiment with . Plus there were about 3 years ago only 10 - 15 people in germany that were allowed to use THC as a medicine . Can someone please work that out ? I mean 1 ton divided by the number of people in germany , the amount one needs to get zapped and then days . Sounds to me like germany would be permanently floating comatose about 3 foot over the ground ......
As far as how they get the genes into the microorganisms goes they use three diferent words = plant , insert and bring . Other than that i cant see any more info about it .