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Author Topic: Tryptamine-Injected Substrates  (Read 166 times)

Moolybdenum

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Tryptamine-Injected Substrates
« on: May 23, 2011, 04:38:20 AM »
I didn't see a thread regarding this fascinating subject of adding tryptamines to the substrate feeding growing mycelium at the forums.

Some research has been done, but not enough.   ;)

A person I talked to about this was quite intrigued by the ideas shared, and also happened to have some DMT and some oh-so precious and amazing DiPT in limited quantities.

He then went on to speak of strange things that I, holding no major knowledge in areas of shroom growin', thought sounded strange but fantastic;  Bird seed, PC's, popcorn, certain psi settings, PR's, perelite, spore AND tryptamine innoculations, mist, and TiT's.   ;D

He said he'd share some more details as time went on.

But this thread doesn't have to be a grow log for one project.  I'd like to see what else may be known from the others here. 

Perhaps there is some new gem held by some one here that I have not seen yet?   :o  One can only hope to learn more and more. . . . . . :)

aniracetam

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Re: Tryptamine-Injected Substrates
« Reply #1 on: May 23, 2011, 06:06:27 PM »
done it, using 10 mM trypt hcl
 BRF substrate, wild-type strain (cubensis) from Bastrop county, maintained on MEA.

the result was cubes which bruised india ink black, and 3 g trips that felt closer to 6.
very potent doped cubes.
« Last Edit: May 23, 2011, 06:15:12 PM by aniracetam »
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." - Max Planck

Vesp

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Re: Tryptamine-Injected Substrates
« Reply #2 on: May 24, 2011, 03:12:34 AM »
Wow that is impressive... any pictures comparing the doped cube bruises and the natural? Very interested in seeing those if you got them.

Adding Tween to these would probably help them take in the tryptamine... same story, different fungi - works with ergot... right?


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aniracetam

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Re: Tryptamine-Injected Substrates
« Reply #3 on: May 24, 2011, 01:54:10 PM »
yep

man...i had those pics  a year ago, but I got rid of them    :-\
wish I still had them. the difference was remarkable... control group bruised azure, experimental group bruised tattoo black.
« Last Edit: May 24, 2011, 01:56:58 PM by aniracetam »
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." - Max Planck

Vesp

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Re: Tryptamine-Injected Substrates
« Reply #4 on: May 24, 2011, 07:19:36 PM »
As discussed elsewhere on this board, you can use this trick to produce unique drugs - varying in potency... having certain substitutions on the tryptamine ring and than adding it to the organism can turn non-psychedelic things into psychedelic things. http://127.0.0.1/talk/index.php/topic,1841.msg19110.html#msg19110 a bit is mentioned here...

This is also supposed to work with other organisms such as cacti, though I have heard no conclusive results about that; has anyone?

.. this thread also is kind of relevant... http://127.0.0.1/talk/index.php/topic,1048.0.html
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Sedit

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Re: Tryptamine-Injected Substrates
« Reply #5 on: May 24, 2011, 08:42:32 PM »
This should work pretty well on Panaeolus foenisecii as well right? If the potancy of these little things could be brought on par with that of the cubensis family then it would be a huge leap forward considering there small size and rapid growth, not to mention they grow all over freshly cut lawns during the right time of year. I ate some of these the other day and it was nice.
There once were some bees and you took all there stuff!
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Vesp

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Re: Tryptamine-Injected Substrates
« Reply #6 on: May 24, 2011, 11:01:30 PM »
http://en.wikipedia.org/wiki/Panaeolina_foenisecii

Not psychoactive.. It is sometimes mistaken for the hallucinogenic Panaeolus subbalteatus or Panaeolus castaneifolius which both share the same habitat and can be differentiated by their jet black spores.

Should work on any mushroom that produces alkaloids related to the type the substrate is doped with...

Those lawn mushrooms are harder to grow than the cubes from what I have read, and in the lab the biological yield is much less than that of the cubes.

But correct me if I am wrong.

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OoBYCoO

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Re: Tryptamine-Injected Substrates
« Reply #7 on: May 24, 2011, 11:16:03 PM »
Basing your conclusion on the bruising doesn't make it valid.  I've grown tons of shrooms and some of my grows on regular brf substrate, depending on the specie/strain, have bruised black b/c the psilocybin content was higher.  The only way to know for sure w/ absolute certainty is w/ a chemical assay.  In your experiment, what was your inoculant?  For your control group and test group to have been a valid comparison you would have had to do agar work resulting in a "true" isolation (no sectoring) of the strain so both groups were grown w/ identical genetics.  Any other method would have been useless and the results can be thrown out the window.  My 2 cents.

aniracetam

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Re: Tryptamine-Injected Substrates
« Reply #8 on: May 25, 2011, 12:04:42 AM »
I also analyzed with nLC-MS, and confirmed a strong signal for psilocin (sample extracted with 0.1% AcOH in HPLC-grade water), better than your typical chemical assay and TLC plates.
Son, I'm piracetam from the shroomery. look at the title below my un.. I analyze chems, quantitatively and qualitatively.

and it was agar work.. pure strain, same plate; no sectors. innoc method was a myc syringe prepared
with sterile water, and myc from said plate.
« Last Edit: May 25, 2011, 02:34:45 AM by aniracetam »
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." - Max Planck

Sedit

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Re: Tryptamine-Injected Substrates
« Reply #9 on: May 25, 2011, 01:50:26 AM »
Quote from: Vesp on May 24, 2011, 11:01:30 PM
http://en.wikipedia.org/wiki/Panaeolina_foenisecii

Not psychoactive.. It is sometimes mistaken for the hallucinogenic Panaeolus subbalteatus or Panaeolus castaneifolius which both share the same habitat and can be differentiated by their jet black spores.

Should work on any mushroom that produces alkaloids related to the type the substrate is doped with...

Those lawn mushrooms are harder to grow than the cubes from what I have read, and in the lab the biological yield is much less than that of the cubes.

But correct me if I am wrong.



I have been reading the same all day and unless I have been eating ones that I thought where them but are really the look alikes that are psychoactive then I disagree with what I have been reading. I have been harvesting these things for years and I can assure you what i'v been eating are active... sadly I just can't assure you that they are not some look alike of the hay makers..
There once were some bees and you took all there stuff!
You pissed off the wasp now enough is enough!!!

OoBYCoO

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Re: Tryptamine-Injected Substrates
« Reply #10 on: May 25, 2011, 04:01:11 AM »
Quote from: aniracetam on May 25, 2011, 12:04:42 AM
I also analyzed with nLC-MS, and confirmed a strong signal for psilocin (sample extracted with 0.1% AcOH in HPLC-grade water), better than your typical chemical assay and TLC plates.
Son, I'm piracetam from the shroomery. look at the title below my un.. I analyze chems, quantitatively and qualitatively.

and it was agar work.. pure strain, same plate; no sectors. innoc method was a myc syringe prepared
with sterile water, and myc from said plate.
Well son, how am I supposed to know who you are on the shroomery?  Am I supposed to think piracetam when I see your username HERE as aniracetam?  lulz  Besides, I've never come across you nor even heard of you over there till now.  And just b/c you put "bioanalytical chemist" under your UN doesn't mean anything.... I could put nuclear engineer under mine, doesn't mean I am one.  Why don't you have a TC tag (not that I have one)??????????  Furthermore, did you post your methods/procedures prior to this post?  Is ANYTHING in my previous post incorrect?  Don't get testy w/ me just b/c your a mod on shroomery (I'm a mod on other sites, big deal), have an impressive self PROclaimed title under your UN, and didn't give any details prior to this post.  Why do you think I asked you a question?  Seriously, am I supposed to be a mind reader and have known this PRIOR to you posting it????????  Really dude?!?!

Look I have no problems w/ you, but no need to act like the almighty dick.  I'm on shroomery too w/ the same UN, holla at me.  ;)

OoBYCoO

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Re: Tryptamine-Injected Substrates
« Reply #11 on: May 25, 2011, 04:04:03 AM »
BTW, just got back home from a wake for a family member and I might've taken it out on you.  Seriously though I have no problems w/ you.

aniracetam

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Re: Tryptamine-Injected Substrates
« Reply #12 on: May 25, 2011, 01:28:55 PM »
no biggie :)

all I'm sayin is...it works.
just gotta find a good concentration that doesn't overload the fungal enzymes

i actually am a bioanalytical chemist, mod at the shroomery is just a privilege.
I work with ESI-MS, MALDI-TOF, capillary electrophoresis, gel electrophoresis, western blot,
and fluorimeters (heaviest concentration on proteomics and mass spec).
have my own spectrophotometer, and LC-UV system, and I use them regularly.
 
shit, I'd actually like to seem more people experiment with these things too. everyone likes to theorize, which is all fun and good, but we gotta get more experiments going   ;D
« Last Edit: May 25, 2011, 01:42:53 PM by aniracetam »
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." - Max Planck

OoBYCoO

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Re: Tryptamine-Injected Substrates
« Reply #13 on: May 25, 2011, 07:43:10 PM »
Agreed!  Pushing the envelope is the only way we can get new discoveries.

Sedit

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Re: Tryptamine-Injected Substrates
« Reply #14 on: May 25, 2011, 08:57:15 PM »
What other materials could work here? Tryptophan should proceed pretty well also I would think correct?
There once were some bees and you took all there stuff!
You pissed off the wasp now enough is enough!!!

Vesp

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Re: Tryptamine-Injected Substrates
« Reply #15 on: May 25, 2011, 09:31:18 PM »
I would think that would work fine as well, and is likely more OTC. might have some advantages/disadvantages though.
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letters

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Re: Tryptamine-Injected Substrates
« Reply #16 on: May 26, 2011, 06:00:13 PM »
iirc the papers showing tryptamine incorporation into the mushrooms showed that tryptophan was not suitable!

aniracetam

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Re: Tryptamine-Injected Substrates
« Reply #17 on: May 26, 2011, 07:46:27 PM »
right
because the mushrooms produce most of their own tryptophan. however, the decarboxylation is a
rate-limiting step, so supplementation with tryptamine bypasses this catabolic bottleneck.
supplementing with NMT gives even better yields of alkaloids
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Vesp

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Re: Tryptamine-Injected Substrates
« Reply #18 on: May 28, 2011, 11:17:37 PM »
What limits the decarboxylation?
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aniracetam

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Re: Tryptamine-Injected Substrates
« Reply #19 on: May 28, 2011, 11:49:48 PM »
net gibbs free energy in the rxn
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." - Max Planck
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