Author Topic: Enemies of submerged culture  (Read 349 times)

oldguy

  • Larvae
  • *
  • Posts: 25
Re: Enemies of submerged culture
« Reply #20 on: June 17, 2011, 12:30:18 AM »
Vesp has this one right, if necessary get a 4' by 2' HEPA filter from one of the mushroom supply companies and a 1600+ cfm fan from Dayton and build your own.  Much easier and better than a glove box.

80% isopropyl alcohol also works well for sterilization.

Vesp

  • Administrator
  • Foundress Queen
  • *****
  • Posts: 3,130
Re: Enemies of submerged culture
« Reply #21 on: June 17, 2011, 04:24:14 AM »
a 24" by 12" Laminar flow hood can be had for close to $600 from a fungi. website.

60% alcohol is the best for sterilisation, higher or lower leads to it not working as well so I was told by a microbiology professor.
Bitcoin address: 1FVrHdXJBr6Z9uhtiQKy4g7c7yHtGKjyLy

overunity33

  • Subordinate Wasp
  • ***
  • Posts: 218
Re: Enemies of submerged culture
« Reply #22 on: June 17, 2011, 05:18:42 PM »
Flow hoods are crazy expensive, I would just use a glovebox.  Maybe a little more restrictive but for like 580$ cheaper you can't complain.  60% alcohol is the perfect ratio between fast killing contams and evaporating away too fast, can get really annoying when you set up to do a procedure and then your 99% iso evaporates off before you can wipe down what you need to..

oldguy

  • Larvae
  • *
  • Posts: 25
Re: Enemies of submerged culture
« Reply #23 on: June 18, 2011, 12:05:35 AM »
They are wicked expensive, almost $1600 plus shipping for the 4 x 2 model, but if you have the woodshop, skills and desire, you can build one as nice or nicer for about half the price.  Glove boxes do work for cheaper, although I wouldn't recommend them for spawn bags.  The big All-American pressure cookers aren't cheap either, particularly the electric version.

I mentioned 80% isopropyl because that's what Paul Stamets recommended to me about a decade ago when I and some others had an extended opportunity to pick his brain.  I'll have to bring it up next time I talk to him.  70% is over the counter where I live, so 60% would be more convenient.

aniracetam

  • bioanalytical chemist
  • Subordinate Wasp
  • ***
  • Posts: 123
Re: Enemies of submerged culture
« Reply #24 on: June 18, 2011, 05:43:25 PM »
sure would.
if you wanted to prepare 200 mL of 60%, you'd just mix 171.43 mL 70% IPA with 28.57 mL DI water
"Experiments are the only means of knowledge at our disposal. The rest is poetry, imagination." - Max Planck

Oerlikon

  • Dominant Queen
  • ****
  • Posts: 365
Re: Enemies of submerged culture
« Reply #25 on: June 19, 2011, 05:22:59 PM »
IS 60-80% best ratio for any alcohol or just IPA!?
Where I live,IPA is very hard to come by,denaturated EtOH in other is very cheap and common.
Welcome to my lab,
where you can choose your own dreams!

fresh1

  • conspirator
  • Dominant Queen
  • ****
  • Posts: 339
Re: Enemies of submerged culture
« Reply #26 on: November 13, 2011, 05:32:56 AM »
Just a thought but what about microwave sterilisation of ?

http://www.labint-online.com/featured-articles/microwave-sterilisation-of-foods-an-industry-changing-development/index.html

I miss TLS' challenging pov's, playing "the devils advocate" is not altogether a bad thing, BUT, i think some of the "problems" he poses are anachronistic (outdated)

 I think what everyone has postulated here is great, there are many ways to the same result in science and chemistry,, it just takes some "thinking outside the square" and some effort.....toady, i think you forgot to add that many peeps are just plain LAZY...not that I have anyone here in mind..... ;)

 
"Curiosity is a gift"

Vesp

  • Administrator
  • Foundress Queen
  • *****
  • Posts: 3,130
Re: Enemies of submerged culture
« Reply #27 on: November 13, 2011, 05:42:56 AM »
Microwave sterilization is probably not very useful as it does not easily allow for you to sterilize the solution. It isn't like you'd just be able to put the liquid in the microwave and boil it. You need to get it to about 121*C and hold it at that temperature for a long time to ensure endospores have been destroyed. If microwave sterilization were done, it would likely just become an expensive and complicated autoclave or pressure cooker.
The simplicity of a  pressure cooker is preferred, especially for what we are discussing.. or possibly a chemical means if the amount is too large to pressure cook/autoclave.


@Fresh1....
People like TLS, Sake, and fresh1 always seem to be overly excited about the topics we focus on, but often poison their own experiences and others  with being too philosophical, outspoken, active and off topic. Sometimes it is best to just read and only comment on topics when you have something that is related and truly useful to the discussion. Its great to question the status quo, authority, and whatever else but it isn't always appropriate and it is detrimental to this site as it can and almost always stifles the real meat & bones of the conversation.
Bitcoin address: 1FVrHdXJBr6Z9uhtiQKy4g7c7yHtGKjyLy