Author Topic: Antibiotics and mushrooms  (Read 117 times)

zajcek01

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Antibiotics and mushrooms
« on: February 04, 2009, 10:51:52 PM »
Here we could success about the use of different antibiotics for mushroom cultures.

I have experimented with psilocybe cubensis and chloramphenicol on PDA and found out, that cultures did not grow very fast, and spores did not germinate at all. A piece of mushroom tissue was added to PDA with chloramphenicol, and it grew very slowly, strandy growths were very thin, only seen under magnifying glass.

At the same time a sample from the same tissue was added to PDA with H2O2 and ordinary PDA without any additives. The both samples were fully overgrown when the growth of the sample with chloramphenicol was still hard to see.

PDA=Potato Dextrose Agar

Do you have any experience using antibiotics during the first stage of mushroom growing/cloning?
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lqdtrance

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Re: Antibiotics and mushrooms
« Reply #1 on: February 05, 2009, 09:12:11 PM »
Forgive my ignorance but why would you want to introduce an antibiotic to a mushroom grow? To fight off contamination's?

zajcek01

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Re: Antibiotics and mushrooms
« Reply #2 on: February 19, 2009, 02:15:48 PM »
Yes, to fight contaminations (bacteria) when using spore prints from wild mushrooms.
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lqdtrance

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Re: Antibiotics and mushrooms
« Reply #3 on: February 19, 2009, 05:27:53 PM »
I see. I would be interested in finding out as well. I hunt mostly and have a huge stock of wild prints. I should attempt a trial using what you describe.
So, in your findings, the agar with the antibiotics didn't do so hot but the agar without the antibiotics and using H2O2 did well? Have you attempted using any other antibiotics? Don't a lot of antibiotics originate from mold and fungus as it is?
Do you have a tek to make your agar? I know I can UTFSE but I am curious if you do it a certain way. Thanks.

Vesp

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Re: Antibiotics and mushrooms
« Reply #4 on: February 20, 2009, 12:27:18 AM »
Where did you get the Antibiotics? I have a few from previous times I was sick, would they work?
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zajcek01

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Re: Antibiotics and mushrooms
« Reply #5 on: February 27, 2009, 12:03:03 PM »
They were bought from Sigma-Aldrich.
What kind of antibiotic do you have?
Penicillin antibiotic may work. If you have some spores and spare time, please do try.
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Vesp

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Re: Antibiotics and mushrooms
« Reply #6 on: February 28, 2009, 12:10:09 AM »
I will try it with the antibiotics I have when I get the chance. Right now I am trying to get an agar culture going of Agaricus bisporus (button mushroom). I made the spore prints from store bought mushrooms, and didn't bother to sterilize much besides the water and syringe I used to make a spore syringe. I'll see if this gets infected with anything and if it does, I'll use the same syringe and use agar with antibiotics to see if it prevents it. Hopefully though, they just won't get infected. :)
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Douchermann

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Re: Antibiotics and mushrooms
« Reply #7 on: March 11, 2009, 07:53:20 AM »
Vesp probably has ciprofloxacin.  That's the new 'wonder antibiotic' the doctors are throwing around, while the chemists work feverishly to make a new antibiotic since it wont be long till things are immune to this.  I would give it a shot regardless and see what happens.  Don't be too liberal with the H2O2 either, it slows down the mycellium right at it's most important growth stage.