Awesome. Any results thus far?
Re: Crude Colchicine Extraction
« Reply #20 on: January 06, 2011, 08:10:21 PM »
Topic: Crude Colchicine Extraction (Read 402 times)
Vesp
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OoBYCoO
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Re: Crude Colchicine Extraction
« Reply #21 on: January 06, 2011, 10:29:16 PM »
Not for me yet. I've decided to wait till spring so I can sow the seeds outside, giving me a higher margin of success... hopefully. 
Oerlikon
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Re: Crude Colchicine Extraction
« Reply #22 on: January 07, 2011, 12:11:14 AM »
Pardon my ignorance,but is colchicine used to make insanely large plants or what!?
Tsathoggua
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Re: Crude Colchicine Extraction
« Reply #23 on: January 07, 2011, 10:50:58 PM »
I believe it has been successfully used in a study on ergot, where it really increased vigor of growth.
I believe it to increase alkaloid production in that fungus also, because that is dependent upon heterokaryotic strains.
I do not have the ref though, I didn't bother compiling it into my notes, as that was the sole bit of info useful in it, it was just a radioligand feeding assay assessing radioactive glucose and acetate incorporation.
I believe it to increase alkaloid production in that fungus also, because that is dependent upon heterokaryotic strains.
I do not have the ref though, I didn't bother compiling it into my notes, as that was the sole bit of info useful in it, it was just a radioligand feeding assay assessing radioactive glucose and acetate incorporation.
thallium
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Re: Crude Colchicine Extraction
« Reply #24 on: February 04, 2011, 05:09:25 PM »
I went out and dug these up from the garden. Are they enough??... Mind you that I'm not looking to make a large amount of the solution.
Those are Crocus bulbs, not Colchicum bulbs, so they contain no colchicine.
ETA: Vesp; your bulbs are also Crocus vernus, you need Colchicum bulbs for this to work.
OoBYCoO
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Re: Crude Colchicine Extraction
« Reply #25 on: February 04, 2011, 05:19:41 PM »
from wiki
Other uses of the name crocus
Though some true crocus bloom with the fall (autumn) rains, after summer's heat and drought, the name autumn crocus is often used as a common name for Colchicum, which is in the lily family (Liliaceae), and which has six stamens; it is also known as meadow saffron, though unlike true saffron the plant is toxic. The so-called prairie crocus (formerly Anemone patens, now Pulsatilla patens or P. ludoviciana) belongs to the buttercup family (Ranunculaceae).
Other uses of the name crocus
Though some true crocus bloom with the fall (autumn) rains, after summer's heat and drought, the name autumn crocus is often used as a common name for Colchicum, which is in the lily family (Liliaceae), and which has six stamens; it is also known as meadow saffron, though unlike true saffron the plant is toxic. The so-called prairie crocus (formerly Anemone patens, now Pulsatilla patens or P. ludoviciana) belongs to the buttercup family (Ranunculaceae).
thallium
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Re: Crude Colchicine Extraction
« Reply #26 on: February 04, 2011, 05:37:41 PM »
I'm talking about Crocus in the botanical sense. You both used Crocus bulbs which contain no colchicine, Colchicum-bulbs are considerably larger and have a different tunic. Trust me, I'm very familiar with both genera, it's my day job...
OoBYCoO
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Re: Crude Colchicine Extraction
« Reply #27 on: February 04, 2011, 05:42:41 PM »
Also from wiki...
Colchicum autumnale, commonly known as autumn crocus, meadow saffron or naked lady, is a flower which resembles the true crocuses, but flowering in autumn. (This is not a reliable distinction, however, since there are many true crocuses that flower in autumn.) The name "naked lady" comes from the fact that the flowers emerge from the ground long after the leaves have died back.
This is the only species of Colchicum native to the United Kingdom, with notable populations under the stewardship of the County Wildlife Trusts.
Toxicity for medicinal uses
The plant has been mistaken by foragers for ramsons, which it vaguely resembles, but is a deadly poison due to the presence of colchicine, a useful drug with a narrow therapeutic index. The symptoms of colchicine poisoning resemble those of arsenic and there is no antidote. Despite its toxicity, colchicine is approved by the US FDA for the treatment of gout and Familial Mediterranean Fever and is also used in plant breeding to produce polyploid strains.
Colchicum autumnale, commonly known as autumn crocus, meadow saffron or naked lady, is a flower which resembles the true crocuses, but flowering in autumn. (This is not a reliable distinction, however, since there are many true crocuses that flower in autumn.) The name "naked lady" comes from the fact that the flowers emerge from the ground long after the leaves have died back.
This is the only species of Colchicum native to the United Kingdom, with notable populations under the stewardship of the County Wildlife Trusts.
Toxicity for medicinal uses
The plant has been mistaken by foragers for ramsons, which it vaguely resembles, but is a deadly poison due to the presence of colchicine, a useful drug with a narrow therapeutic index. The symptoms of colchicine poisoning resemble those of arsenic and there is no antidote. Despite its toxicity, colchicine is approved by the US FDA for the treatment of gout and Familial Mediterranean Fever and is also used in plant breeding to produce polyploid strains.
thallium
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Re: Crude Colchicine Extraction
« Reply #28 on: February 04, 2011, 05:47:26 PM »
Also from wiki...
Colchicum autumnale, commonly known as autumn crocus, meadow saffron or naked lady,
Your point being? You both used corms of the genus Crocus, which contain no colchicine. Google images: "Crocus vernus", "Crocus corm", "Colchicum" and "Colchicum bulb", and you'll see that I'm right.
For those of you living in warmer regions I believe Gloriosa contain as much, if not more, colchicine in it's bulbs than Colchicum.
thallium
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Re: Crude Colchicine Extraction
« Reply #29 on: February 04, 2011, 05:55:44 PM »
I did the work for you, below are links to images that clearly show the difference between Colchicum and Crocus bulbs - Colchicum have shots that sprout from the base of the bulb, while Crocus have shots that sprout from the center of the corm. (Colchicum bulbs are also much larger, often 10cm long, with a solid (not fibrous, think onion) tunic.
Colchicum bulbs
Crocus corms
Colchicum bulbs
Crocus corms
thallium
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Re: Crude Colchicine Extraction
« Reply #30 on: February 04, 2011, 06:20:00 PM »
...and those of you living in the states can use the herbicide Surflan (oryzalin) instead of colchicine to induse polyploidy, it's much safer and is supposed to ahve a much higher success rate.
See for example:
http://refhide.com/?http://www.forum.haszysz.com/novel-method-inducing-polyploidy-rhododendron-seedlings-t32039.html
http://refhide.com/?http://members.tripod.com/h_syriacus/tetraploidy.htm
See for example:
http://refhide.com/?http://www.forum.haszysz.com/novel-method-inducing-polyploidy-rhododendron-seedlings-t32039.html
http://refhide.com/?http://members.tripod.com/h_syriacus/tetraploidy.htm