Doing the required bookworming to further my interest in a viable ergot bioreactor culture, I decided on immobilized cell culture in (probably) alginate microspheres, one problem is, with alginate immobilisation, after about 150 uM into the beads, it becomes an almost completely anoxic environment, and of course production of valuable lysergic acid and lysergyl peptides uses enzymes that need atmospheric oxygen to perform oxidations requisite to the biosynthesis of these alkaloids.

Perfluorocarbons of various kinds have been used in the form of emulsions localised within the beads, resulting in some cases in as much as a 7% increase in ergotamine production, as well as mixed into the culture medium, I intend to do both, question is....which perfluorocarbon should I use?

I have noted perfluoroctyl bromide, and hostinert type fluorinated polyethers used, with variable results, but as yet, I can't find a comparitive study attempting to determine the optimum perfluoro compound used.