Ive been pondering. Say I got a ref which has rF values for my product on a certain silica gel type (like type G, H, different mesh sizes, etc...).
If I was to replace that type with another, would the rF values change as well?
More than likely yes, any time you change any aspect of the system it is possible to realize a change in rf, and many times if you try to reproduce exactly what you have done with the same exact system you may still see a change in RF Your analyte could have affinity/interaction (bad terminology I know) with Gypsum, therefore rf could be different between a G and H plate. If you change the mesh size you will also more than likely experience a change in rf. As far as I am concerned the only way to more accurately qualitatively analyze your separation is by running a known standard, under optimized conditions. But then again I can't see myself ever running a TLC plate for any reason, so I guess you can take this advice with a grain of salt. Hopefully there are some TLC experts lurking around here, (I am sure there are) that can give you a better practical explanation than I.
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Your analyte could have affinity/interaction (bad terminology I know) with Gypsum, therefore rf could be different between a G and H plate. If you change the mesh size you will also more than likely experience a change in rf. As far as I am concerned the only way to more accurately qualitatively analyze your separation is by running a known standard, under optimized conditions. But then again I can't see myself ever running a TLC plate for any reason, so I guess you can take this advice with a grain of salt. Hopefully there are some TLC experts lurking around here, (I am sure there are) that can give you a better practical explanation than I.