Ergot, Claviceps & Lysegic Acid derivs.

CherrieBaby · chouchou

Production of Ergot Alkaloids of Lysergic Acid Derivatives (LAD) by Submerged Fermentation
K. S. M. Sastry, A. Ojha, C. K. Atal
Indian Drugs, January, 1979, pp 88-93.
Abstract: Out of 100 isolates of ergot fungi belonging to different chemical races screened, only very few have shown their capacity to produce alkaloids under submerged fermentation. Out of these, three isolates of Claviceps paspali Stevens and Hall are able to produce considerably in high concentration, the lysergic acid derivatives (LAD) in mannitol succinic acid medium containing eight inorganic salts. Age of the initial and seed culture, percentage of inoculum, homogenisation time, duration of fermentation cycle in the production medium and finally the recovery of total LAD produced are some of the parameters standardised for one high yielding isolate of ergot fungus.

http://rapidshare.de/files/1059817/Ergot_Indian.Drugs.1979.pp88-93.pdf.html
(PDF, text, 77 Kb)

CherrieBaby · chouchou

Life History and Poisonous Properties of Claviceps Paspali
H. B. Brown
Journal of Agricultural Research, v. 7, pp. 401-407 (1916)

http://rapidshare.de/files/1108662/Claviceps.paspali_J.Agr.Res.v7.pp401-7_1916_.pdf.html
(PDF, text, 684 Kb)

CherrieBaby · chouchou

6-Methyl-Δ8,9-ergolen-8-carbonsäure, ein neues Ergolinderivat aus Kulturen eines Stammes von Claviceps paspali Stevens et Hall). Mitteilung über Mutterkornalkaloide.
H. Kobel, E. Schreier, J. Rutschmann
Helvetica Chimica Acta, 1964, Vol. 47, pp. 1052-1064

http://rapidshare.de/files/1138173/Claviceps.paspali_Helv.Chim.Acta.47.1964.pp1052-64_Txt-Deutsch.pdf.html
(190 Kb, PDF file, German. OCRed text. Scanned at 600 dpi, OCRed using the ABBYY German Medical dictionary. The only mistake I'll aware of is that I had to use images for the triple = sign (HTML &equiv;). These happen only 3 times in the text [search for N+ to find them])

Comment: 3 races of Claviceps paspali all generally produce more clavine alkaloids, in submerged culture, than C. Purpurea. It was fairly easy to isolate a strain capable of producing 1.5 g/L of Paspalic acid derivitives. Paspalic acid is easily isomerised to Lysergic acid.

CherrieBaby · chouchou

Low-Cost Fermentor
Andrew S. Hopkins
Journal of Chemical Education, 71, 1994, pp. 529-530

http://rapidshare.de/files/1143677/Low-Cost.Fermentor_J.Chem.Ed.71.pp529-530.1994.mht.html
(37Kb, HTML, MS Web-archive)

Comment: How to build a 1-2 Litre Fermentor for next to nothing (Note. - requires an autoclave which does not cost next to nothing)

This seems like an appropriate place to put this stuff.

0xYg3n · Fri Apr 08, 2005 9:39 am

awesome stuff, thx.

MargaretThatcher · Fri Apr 08, 2005 5:39 pm

http://img105.exs.cx/my.php?loc=img105&image=lowcostfermentorp1of24ff.gif
(114 KB)

Page 2 is missing from their electronic archive.

Ionium · Mon Aug 22, 2005 1:54 am

All the files supplied by CherrieBaby has timed out, unfortunately. If anyone has them, I'd be very happy if they somehow could make them available again.

Thank in advance.

nooneinparticular · Mon Aug 22, 2005 3:02 am

I've got the article in the first post. Unfortunately, it was the only one I cared to download.

http://rapidshare.de/files/4227778/Ergot_Indian_1_.Drugs.1979.pp88-93.pdf.html