[/quote]The final fermentation broth contains a complex mixture of alkaloids, salts, polysaccharides, fats, solids, etc., and organic solvent extraction is commonly used for the separation of ergot alkaloids from the broth. To avoid the formation of emulsions during the extraction, a separation process taking advantage of solid-liquid adsorption of alkaloids on activated carbon bentonite and other silicate sorbents was developed in 1986 [7]. In 1988 selective adsorption of polycarboxylic ester resin XAD-7 to isolate alkaloids[/quote]

http://www.world-of-fungi.org/Mostly_Medical/Ziad_Madlom/Ergot_alkaloids.htm

also interesting is it is possible to isolate lysergamides from the biomass of morning glory vines (leaves stems roots etc.)

according to this old hive thread in the post here:

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/2213476

the problem with low yeilding biomass is scale, the solution is absorption to extract large quantites.
and since morinig vines grow everywhere and all species have some lysergamides you can't go wrong.

i tried some dried ipomea violacea leaves last night and got a slight lsd like buzz id say under 50 mic's but it was noticable.
there are goods in the morning glory vine for sure.
i ingested maybe 3 grams dried weight at the most i'd say the leaf content is 1/2-1/3'rd of that of the seeds it definitely suprized me.
so a kilogram of dried morning glory vine could concievably contain between 100-200 milligrams of lysergamides.
a high throughput method of extracting biomass that contains negligible amounts of ergolines would make obtaining these alkaloids much more practicable.

 as to methanol it's dirt cheap who really cares?
just rotovap it and recycle it.
that thread just prooves (along with my bioassay) there are *some* lysergic goodies in the morning glory vines and leaves.
making lysergic acid  easy to come by for the expirimenter is the goal.

here is a paper describing the isolation and characterization of alkaloids from genus ipomea
foilage

http://www.erowid.org/references/refs_view.php?A=ShowDocPartFrame&ID=7684&DocPartID=6799

Can you guys speculate as to which alkaloids this method would tend to extract? LSA has primary, secondary and tertiary amines, which would be more prone to being adsorbed?

Most interesting.

Although you are mixing up your herb lore a little....sort of

These are not nightshades. The morning glory, ololiuqui, HBWR, etc, they are members of the convolvulacea, the bindweeds. You might see a relative of them out in the wild, or as a weed, they are in the order Solanales, this is not the Solanacea though Solanacea is the family, as is the Convolvulacea, the order, is one step up in the ranking, so to speak. Going closer and closer to the classification, the further removed you get, I.e Plantae, the kingdom of plants, and back a couple to the prokaryotes, eukaryotes and the funky ass archea.

The nightshades, belladonna, Daturas, Brugmansias, etc. are in the family (not order) Solanacea, within the Solanales. As are tomatoes, the potato, aubergine/eggplant, quite surprises some people how many things related to the (im)famous deadly nightshade/belladonna they are actually eating:D

Jalap and scammony are usually obtained from the roots of the plants (I speak of the drug jalap, and scammony, as resin scammoniae, rather than the plants themselves here) by alcoholic extraction, or slashing the roots although the latter produces an inferior product. Interestingly, I just read, that they are inactive via IV, or any other route than per os, their undergoing a reaction with compounds in the bile, one of them being taurocholate, to form the purgative compound. The scammonins are in and of themselves, inactive, acting on the digestive tract only after this metabolic transformation.

So I daresay, even if they had been purgative, plugging an extract would have allowed it to be used without....undesirable properties....being called upon. 

Interesting....

The morning glory grows pretty quickly too, and can become a weed. Hah, have that, you government bastards!

With that bentonite extraction procedure floating about...oh boy....

Just be careful of the ident.

There are two very toxic plants with heart-shaped leaves! The black, and the white bryony. Although the flowers are completely different. One of them is a member of the Cucurbitacea, the gourd family, (and cucumbers, courgettes etc, melons, pumpkins), and can be distinguished by much thicker stems, which if I remember from the last time I saw the plant, has the occasional quite stiff, short hair on them. The other does not have tendrils. I forget which is which.

Both of these contain very acrid, irritant poisons, and could quite easily be fatal if ingested.

Wasn't doubting the ID jon, I was warning others that might just grab the first plant with heart shaped leaves they see that twines and curls as a climbing creeper around the objects at hand. Doing that, and getting bryony (either black, or the unrelated white bryony) would quite possibly prove deadly.

The hairs on the one that has them, they are almost spines, they are that stiff, quite sparse in distribution along the stem (as opposed to say, cowhage pods, where they cover it, for instance), indeed, they almost look like large stinging nettle stingers, very stiff, very thick, not many of them.

As far as toxic things tasting bad....not a reliable test. Some people who have eaten the destroying angel (and died horribly) said it tasted pretty good. As far as plants, some do, sure, but plenty of poisonous fruit, for instance out there, that would do you in and probably taste good. And a practical demonstration of the falsehood of that notion-take a small twig of woody (not deadly, not black, woody) nightshade, and chew it. It will appear at first bitter, then taste pleasantly sweet.

Its not highly poisonous, one can do this, without becoming ill, to witness the change, although of course it should not be eaten (it has attractive berries)

 I get a 404 error when I try to link to the "world-of-fungi" page. Does anyone have it downloaded or have access to another place that might have the info? I'd really like to check it out!

  --Sol

 sahsha shulgin makes some interesting comments about LSA, in TIHKAL...

an easier way to go about it is to just hit it with a blacklight at the right frequency you can detect as little as 1 ug of lysergamides just to be sure.

IIRC, One of which is the very destructive nature of UV light on LSA/LSD & co

I have often wondered whether doing procedures of this nature would be best done under IR rather than UV  

 F1

BTW...IR stands for Infra Red, but I was a little off, for what I meant, as Sol said, is the light one would use in a darkroom.

Vesp yr probably right as long as the UV light is indirect, however even with it being less intense than sunlight I do know that UV light are used to render collectable blotter art that's been dipped inert and inactive and thus kosher for legal possession. I'm not sure if a simple blacklight will do this or not though. My understanding was that the optimal lighting to prevent damage to ergolines was something like a red photographic darkroom light.

 Still can't get that 'world-of-fungi' page Jon linked to in the OP-- does anyone have the info from that link??

  --Sol